Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27413 | 3' | -49.4 | NC_005869.1 | + | 9361 | 0.67 | 0.857208 |
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Target: 5'- --cGGGGACgGGGGGcgcaggccggGGACGgCGCCg -3' miRNA: 3'- guaCCUCUGgUUUCCa---------UUUGUgGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 6464 | 0.67 | 0.857208 |
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Target: 5'- uGUGGAggguGACCAcguccacgGAGGUGGcuaccuCGCCGCg -3' miRNA: 3'- gUACCU----CUGGU--------UUCCAUUu-----GUGGCGg -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 20403 | 0.67 | 0.866035 |
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Target: 5'- cCAUGGAGAUCAAucu----CACgGCCa -3' miRNA: 3'- -GUACCUCUGGUUuccauuuGUGgCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 9263 | 0.67 | 0.848106 |
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Target: 5'- --cGGGGGCguGcuGGGUccGGcCACCGCCg -3' miRNA: 3'- guaCCUCUGguU--UCCA--UUuGUGGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 15061 | 0.67 | 0.838741 |
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Target: 5'- gGUGGuGACCccGAGGGUGcuCAgCaGCCg -3' miRNA: 3'- gUACCuCUGG--UUUCCAUuuGUgG-CGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 1957 | 0.68 | 0.809177 |
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Target: 5'- gAUGcGGGACCAGGuGGUGcccaAGCGcCCGCg -3' miRNA: 3'- gUAC-CUCUGGUUU-CCAU----UUGU-GGCGg -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 26568 | 0.69 | 0.766804 |
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Target: 5'- --cGGAGuaGCCAGccAGGUAgGACACCuCCg -3' miRNA: 3'- guaCCUC--UGGUU--UCCAU-UUGUGGcGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 6790 | 0.77 | 0.304844 |
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Target: 5'- --aGGAGGCCGAugaaguugGGGUAgcAGCGgCCGCCg -3' miRNA: 3'- guaCCUCUGGUU--------UCCAU--UUGU-GGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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