Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27413 | 5' | -61.2 | NC_005869.1 | + | 15214 | 0.66 | 0.317385 |
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Target: 5'- uACGGGGGCgCCCgcCGcCU---GUCCCc -3' miRNA: 3'- -UGCCCCCGgGGGa-GCaGAagcUAGGG- -5' |
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| 27413 | 5' | -61.2 | NC_005869.1 | + | 16184 | 0.68 | 0.241278 |
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Target: 5'- aGCGGcGGGCccgCCCCUCGcCgaccccCGcGUCCCg -3' miRNA: 3'- -UGCC-CCCG---GGGGAGCaGaa----GC-UAGGG- -5' |
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| 27413 | 5' | -61.2 | NC_005869.1 | + | 21049 | 0.68 | 0.235171 |
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Target: 5'- cAUGaGGcGGCCCuCCUCGUCgcugUCaaaggcGUCCCa -3' miRNA: 3'- -UGC-CC-CCGGG-GGAGCAGa---AGc-----UAGGG- -5' |
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| 27413 | 5' | -61.2 | NC_005869.1 | + | 8228 | 0.69 | 0.206559 |
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Target: 5'- gGCGGcGGGCCCUCgggCGggg--GGUCCCa -3' miRNA: 3'- -UGCC-CCCGGGGGa--GCagaagCUAGGG- -5' |
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| 27413 | 5' | -61.2 | NC_005869.1 | + | 5660 | 0.69 | 0.199105 |
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Target: 5'- -aGGGGGCCCCCguuccgcuugaggCGguguuuacCUUUGGUCuCCa -3' miRNA: 3'- ugCCCCCGGGGGa------------GCa-------GAAGCUAG-GG- -5' |
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| 27413 | 5' | -61.2 | NC_005869.1 | + | 24554 | 0.73 | 0.10485 |
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Target: 5'- cGCGGGGGCgCCUUCGagCUgCGGcCCCg -3' miRNA: 3'- -UGCCCCCGgGGGAGCa-GAaGCUaGGG- -5' |
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| 27413 | 5' | -61.2 | NC_005869.1 | + | 23746 | 0.74 | 0.083784 |
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Target: 5'- -gGGcGGGCCCCCUCcUCUUCc-UCCCc -3' miRNA: 3'- ugCC-CCCGGGGGAGcAGAAGcuAGGG- -5' |
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| 27413 | 5' | -61.2 | NC_005869.1 | + | 28389 | 1.09 | 0.000151 |
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Target: 5'- aACGGGGGCCCCCUCGUCUUCGAUCCCc -3' miRNA: 3'- -UGCCCCCGGGGGAGCAGAAGCUAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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