Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27414 | 5' | -57.8 | NC_005869.1 | + | 26520 | 0.66 | 0.427892 |
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Target: 5'- cCGcGCAGGUgCUCGCCcaccgccgcgaGGUGCCCc -3' miRNA: 3'- -GCuCGUCCG-GAGCGGuaa--------UCACGGGu -5' |
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| 27414 | 5' | -57.8 | NC_005869.1 | + | 16623 | 0.66 | 0.40277 |
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Target: 5'- cCGGGCGGGCCggccccagGCCGUcgUGGcGCCgCAg -3' miRNA: 3'- -GCUCGUCCGGag------CGGUA--AUCaCGG-GU- -5' |
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| 27414 | 5' | -57.8 | NC_005869.1 | + | 17862 | 0.66 | 0.393359 |
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Target: 5'- gGGGCGGGCC-CGCCGc---UGCCg- -3' miRNA: 3'- gCUCGUCCGGaGCGGUaaucACGGgu -5' |
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| 27414 | 5' | -57.8 | NC_005869.1 | + | 11747 | 0.66 | 0.384092 |
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Target: 5'- uGAGCGcGGCCUC-CC--UGGcGCCCGa -3' miRNA: 3'- gCUCGU-CCGGAGcGGuaAUCaCGGGU- -5' |
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| 27414 | 5' | -57.8 | NC_005869.1 | + | 16680 | 0.67 | 0.374971 |
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Target: 5'- gGAGCGGGCggCgGCCGcc-GUGCCCc -3' miRNA: 3'- gCUCGUCCGgaG-CGGUaauCACGGGu -5' |
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| 27414 | 5' | -57.8 | NC_005869.1 | + | 21281 | 0.67 | 0.339979 |
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Target: 5'- -cGGCuGGCCUCGUCcagGGcGCCCGu -3' miRNA: 3'- gcUCGuCCGGAGCGGuaaUCaCGGGU- -5' |
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| 27414 | 5' | -57.8 | NC_005869.1 | + | 6044 | 0.69 | 0.26313 |
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Target: 5'- --cGCAGGCCUCGUCAccGGaGUCCGc -3' miRNA: 3'- gcuCGUCCGGAGCGGUaaUCaCGGGU- -5' |
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| 27414 | 5' | -57.8 | NC_005869.1 | + | 17882 | 0.7 | 0.230306 |
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Target: 5'- aGGGguGGCauccUGCCGgcgcUGGUGCCCAu -3' miRNA: 3'- gCUCguCCGga--GCGGUa---AUCACGGGU- -5' |
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| 27414 | 5' | -57.8 | NC_005869.1 | + | 25730 | 0.71 | 0.179949 |
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Target: 5'- gGAGCuGGCCcgCGCCGccGG-GCCCAc -3' miRNA: 3'- gCUCGuCCGGa-GCGGUaaUCaCGGGU- -5' |
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| 27414 | 5' | -57.8 | NC_005869.1 | + | 28150 | 1.08 | 0.000281 |
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Target: 5'- uCGAGCAGGCCUCGCCAUUAGUGCCCAa -3' miRNA: 3'- -GCUCGUCCGGAGCGGUAAUCACGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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