Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27415 | 5' | -49.6 | NC_005869.1 | + | 16647 | 0.66 | 0.859949 |
Target: 5'- cGUGGcgccGCAGcucagcGCGGCCgCGGCGCUGGa -3' miRNA: 3'- -UAUCuu--UGUC------UGUUGGaGUCGCGACU- -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 17281 | 0.66 | 0.841304 |
Target: 5'- -gGGAGGCuGGGCAgcACCUCgGGCGCg-- -3' miRNA: 3'- uaUCUUUG-UCUGU--UGGAG-UCGCGacu -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 21616 | 0.67 | 0.811382 |
Target: 5'- -cAGGAGCAGcaGCAGCCg-GGUGUUGGg -3' miRNA: 3'- uaUCUUUGUC--UGUUGGagUCGCGACU- -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 22485 | 0.67 | 0.800937 |
Target: 5'- --uGGAACAGACAcACCUgcacaaAGCGCUcGAu -3' miRNA: 3'- uauCUUUGUCUGU-UGGAg-----UCGCGA-CU- -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 31077 | 0.67 | 0.800937 |
Target: 5'- uGUAGGAGCAG-CGGCU--GGgGCUGAa -3' miRNA: 3'- -UAUCUUUGUCuGUUGGagUCgCGACU- -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 11814 | 0.67 | 0.779422 |
Target: 5'- uUGGGAGCAGGCucuGCggcgcaUCauGGCGCUGAc -3' miRNA: 3'- uAUCUUUGUCUGu--UGg-----AG--UCGCGACU- -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 15465 | 0.67 | 0.768381 |
Target: 5'- gGUGGAGGCGG-UAGCC-CGGCGCc-- -3' miRNA: 3'- -UAUCUUUGUCuGUUGGaGUCGCGacu -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 6673 | 0.67 | 0.768381 |
Target: 5'- -cAGcgGCGGGCGGCCagGGCGCg-- -3' miRNA: 3'- uaUCuuUGUCUGUUGGagUCGCGacu -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 11772 | 0.68 | 0.757172 |
Target: 5'- -aGGAGGCAGAagaguCCgcaggCGGCGCUGu -3' miRNA: 3'- uaUCUUUGUCUguu--GGa----GUCGCGACu -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 11908 | 0.69 | 0.687211 |
Target: 5'- cUGGAGGCGGugGuGCCUCcGCGCa-- -3' miRNA: 3'- uAUCUUUGUCugU-UGGAGuCGCGacu -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 3763 | 0.7 | 0.639092 |
Target: 5'- -gAGGAGguGGC-GCC-CGGCGCUGGa -3' miRNA: 3'- uaUCUUUguCUGuUGGaGUCGCGACU- -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 2361 | 0.7 | 0.590843 |
Target: 5'- -gGGAGGCGGugAACCUgccCAGCG-UGAg -3' miRNA: 3'- uaUCUUUGUCugUUGGA---GUCGCgACU- -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 24336 | 0.71 | 0.566922 |
Target: 5'- cAUGGAcGCGGACGugCUgCAGCGCc-- -3' miRNA: 3'- -UAUCUuUGUCUGUugGA-GUCGCGacu -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 6118 | 0.75 | 0.370803 |
Target: 5'- gGUGGguACGcuacGACAaaguccggggugACCUCAGCGCUGAg -3' miRNA: 3'- -UAUCuuUGU----CUGU------------UGGAGUCGCGACU- -5' |
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27415 | 5' | -49.6 | NC_005869.1 | + | 27885 | 1.08 | 0.001984 |
Target: 5'- cAUAGAAACAGACAACCUCAGCGCUGAg -3' miRNA: 3'- -UAUCUUUGUCUGUUGGAGUCGCGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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