Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27416 | 3' | -54 | NC_005869.1 | + | 18559 | 0.66 | 0.682818 |
Target: 5'- cGGCGCcgcgcGCGGCggCG-CCGGGCUAc -3' miRNA: 3'- cCUGCGaa---UGCUGuaGCaGGUCCGGU- -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 28114 | 0.66 | 0.66807 |
Target: 5'- uGGACGCggACGACGaggaccUCuGUCuccacacgcucgagCAGGCCu -3' miRNA: 3'- -CCUGCGaaUGCUGU------AG-CAG--------------GUCCGGu -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 7647 | 0.66 | 0.648696 |
Target: 5'- aGGGgGUccuCGGgGUCGUCCggcAGGCCGg -3' miRNA: 3'- -CCUgCGaauGCUgUAGCAGG---UCCGGU- -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 21486 | 0.66 | 0.647555 |
Target: 5'- uGACGCUggugaucuccuggUACGuGCGcUCGUCCAcaugcaaguuGGCCAg -3' miRNA: 3'- cCUGCGA-------------AUGC-UGU-AGCAGGU----------CCGGU- -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 21267 | 0.66 | 0.637274 |
Target: 5'- uGGGCuccaGgUUGCGGCuggccUCGUCCAGGgCGc -3' miRNA: 3'- -CCUG----CgAAUGCUGu----AGCAGGUCCgGU- -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 18689 | 0.66 | 0.637274 |
Target: 5'- gGGugGCgccgGCGAUcgCGUugaCCAcgucGGCCAc -3' miRNA: 3'- -CCugCGaa--UGCUGuaGCA---GGU----CCGGU- -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 22017 | 0.67 | 0.60302 |
Target: 5'- -aGCGCUgcUGCGcCAUCGUCaacacGGCCGc -3' miRNA: 3'- ccUGCGA--AUGCuGUAGCAGgu---CCGGU- -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 6121 | 0.67 | 0.591642 |
Target: 5'- gGGuACGC-UACGACAaaGUCCGGGgUg -3' miRNA: 3'- -CC-UGCGaAUGCUGUagCAGGUCCgGu -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 5403 | 0.69 | 0.501611 |
Target: 5'- uGGCGCUuccgagcUGCG-CGUCGUUCAGGUa- -3' miRNA: 3'- cCUGCGA-------AUGCuGUAGCAGGUCCGgu -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 26481 | 0.69 | 0.501611 |
Target: 5'- uGGGCcacaacaucucggGCUUcgAUGAgAUCGUCCuGGCCGc -3' miRNA: 3'- -CCUG-------------CGAA--UGCUgUAGCAGGuCCGGU- -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 17898 | 0.69 | 0.498381 |
Target: 5'- cGGCGCUggugcccaucaucGCGGCAUCcaUCUGGGCCAu -3' miRNA: 3'- cCUGCGAa------------UGCUGUAGc-AGGUCCGGU- -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 16967 | 0.69 | 0.481317 |
Target: 5'- -cGCGCacggagGCGACcgCGguggCCGGGCCGg -3' miRNA: 3'- ccUGCGaa----UGCUGuaGCa---GGUCCGGU- -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 7050 | 0.7 | 0.439965 |
Target: 5'- cGGGCGgUgACGACGaCGUCCuGGGCgCAg -3' miRNA: 3'- -CCUGCgAaUGCUGUaGCAGG-UCCG-GU- -5' |
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27416 | 3' | -54 | NC_005869.1 | + | 26945 | 1.1 | 0.000624 |
Target: 5'- gGGACGCUUACGACAUCGUCCAGGCCAc -3' miRNA: 3'- -CCUGCGAAUGCUGUAGCAGGUCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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