Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27417 | 5' | -58.2 | NC_005869.1 | + | 16733 | 0.65 | 0.443323 |
Target: 5'- --gGGUCuCGGGAgcggucuggacgccCACCgaggCGCuCCUCAg -3' miRNA: 3'- guaCCAG-GCCCU--------------GUGGaa--GCG-GGAGU- -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 21419 | 0.66 | 0.439369 |
Target: 5'- -uUGGUCaggaagaaguugaGGGugGCCUgcagccgcgccgcgUCGUCCUCGu -3' miRNA: 3'- guACCAGg------------CCCugUGGA--------------AGCGGGAGU- -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 33620 | 0.66 | 0.426659 |
Target: 5'- gAUGG-CCGGuGACuCCUcUCGCCC-CGc -3' miRNA: 3'- gUACCaGGCC-CUGuGGA-AGCGGGaGU- -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 17432 | 0.67 | 0.388963 |
Target: 5'- gGUGGuUCCGGuGGC-CCggCGCCCg-- -3' miRNA: 3'- gUACC-AGGCC-CUGuGGaaGCGGGagu -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 12969 | 0.67 | 0.377191 |
Target: 5'- --cGGcCCGGGACGCCUUUgacaGCgacgaggagggccgCCUCAu -3' miRNA: 3'- guaCCaGGCCCUGUGGAAG----CG--------------GGAGU- -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 4582 | 0.68 | 0.336673 |
Target: 5'- cCGUGG-CgGGGACGgugaguuguCCUUCGCCgUUCAg -3' miRNA: 3'- -GUACCaGgCCCUGU---------GGAAGCGG-GAGU- -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 9523 | 0.68 | 0.320419 |
Target: 5'- --gGGUCCGGGGCGCagguucCGUUCUCc -3' miRNA: 3'- guaCCAGGCCCUGUGgaa---GCGGGAGu -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 276 | 0.68 | 0.297154 |
Target: 5'- -cUGGaCCGG---ACCUUCGCCCUCc -3' miRNA: 3'- guACCaGGCCcugUGGAAGCGGGAGu -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 9448 | 0.7 | 0.248019 |
Target: 5'- gCAUGGUCuCGGuGACGgCgcggcCGCCCUCc -3' miRNA: 3'- -GUACCAG-GCC-CUGUgGaa---GCGGGAGu -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 26582 | 0.7 | 0.229106 |
Target: 5'- --aGGUa--GGACACCUcCGCCCUCGg -3' miRNA: 3'- guaCCAggcCCUGUGGAaGCGGGAGU- -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 9091 | 0.7 | 0.217185 |
Target: 5'- --aGGUCCucgaGGGGCGUC-UCGCCCUCGu -3' miRNA: 3'- guaCCAGG----CCCUGUGGaAGCGGGAGU- -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 4742 | 0.72 | 0.160777 |
Target: 5'- uGUGGUCCGGaccGCugCgguagUUGCCCUCGc -3' miRNA: 3'- gUACCAGGCCc--UGugGa----AGCGGGAGU- -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 12754 | 0.74 | 0.132109 |
Target: 5'- -uUGGUCUGGGcCACCguguaGCCCUCc -3' miRNA: 3'- guACCAGGCCCuGUGGaag--CGGGAGu -5' |
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27417 | 5' | -58.2 | NC_005869.1 | + | 26721 | 1.07 | 0.000367 |
Target: 5'- uCAUGGUCCGGGACACCUUCGCCCUCAc -3' miRNA: 3'- -GUACCAGGCCCUGUGGAAGCGGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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