Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2742 | 5' | -60.5 | NC_001491.2 | + | 65836 | 0.66 | 0.672495 |
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Target: 5'- cGCAA--GCGCCGccaugaCUGGGACGCAa -3' miRNA: 3'- uUGUUacCGCGGCcg----GACCCUGCGUa -5' |
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| 2742 | 5' | -60.5 | NC_001491.2 | + | 147720 | 0.67 | 0.581113 |
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Target: 5'- cGCGAUGGUgGCCagGGCCUGGGgaucgaaggugaGCGCc- -3' miRNA: 3'- uUGUUACCG-CGG--CCGGACCC------------UGCGua -5' |
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| 2742 | 5' | -60.5 | NC_001491.2 | + | 20084 | 0.67 | 0.561021 |
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Target: 5'- cACGcgGGaaCGcCCGGCacggCUGGGGCGCAUg -3' miRNA: 3'- uUGUuaCC--GC-GGCCG----GACCCUGCGUA- -5' |
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| 2742 | 5' | -60.5 | NC_001491.2 | + | 46237 | 0.68 | 0.551045 |
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Target: 5'- aGGCGGUGGCGuCUGGUUUGccGGugGCGg -3' miRNA: 3'- -UUGUUACCGC-GGCCGGAC--CCugCGUa -5' |
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| 2742 | 5' | -60.5 | NC_001491.2 | + | 147400 | 0.68 | 0.541123 |
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Target: 5'- -----aGGCuCCGGCCUGGGcCGCc- -3' miRNA: 3'- uuguuaCCGcGGCCGGACCCuGCGua -5' |
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| 2742 | 5' | -60.5 | NC_001491.2 | + | 148650 | 0.68 | 0.512713 |
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Target: 5'- cAGCGcc-GCGCCGGCCgcaggcgagguacacGGGGCGCAg -3' miRNA: 3'- -UUGUuacCGCGGCCGGa--------------CCCUGCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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