Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27421 | 3' | -52.8 | NC_005869.1 | + | 13240 | 0.67 | 0.634455 |
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Target: 5'- gGCCaGCGGcGccgacagccuGGAGUggGACGCGCUGCu -3' miRNA: 3'- -UGGaUGCU-C----------UCUCAa-CUGUGCGACGu -5' |
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| 27421 | 3' | -52.8 | NC_005869.1 | + | 3678 | 0.68 | 0.587813 |
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Target: 5'- -gCUGCGGGAGguGGUggaGAUACaGCUGCGg -3' miRNA: 3'- ugGAUGCUCUC--UCAa--CUGUG-CGACGU- -5' |
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| 27421 | 3' | -52.8 | NC_005869.1 | + | 9750 | 0.69 | 0.520401 |
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Target: 5'- uGCCggaugGCGcgcaGGAGGGUgaggucuuugcggccGGCGCGCUGCAg -3' miRNA: 3'- -UGGa----UGC----UCUCUCAa--------------CUGUGCGACGU- -5' |
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| 27421 | 3' | -52.8 | NC_005869.1 | + | 1738 | 0.7 | 0.464685 |
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Target: 5'- uACCUGCuGGGGGAGUUGAgcgaGCGCacggGCc -3' miRNA: 3'- -UGGAUG-CUCUCUCAACUg---UGCGa---CGu -5' |
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| 27421 | 3' | -52.8 | NC_005869.1 | + | 6297 | 0.7 | 0.454123 |
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Target: 5'- gGCgUugGAGAGaAGcUUGGCgAUGCUGCGg -3' miRNA: 3'- -UGgAugCUCUC-UC-AACUG-UGCGACGU- -5' |
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| 27421 | 3' | -52.8 | NC_005869.1 | + | 11176 | 0.71 | 0.403368 |
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Target: 5'- uGCCgggACGAGGGcauGGUgaagGAgGCGCUGCu -3' miRNA: 3'- -UGGa--UGCUCUC---UCAa---CUgUGCGACGu -5' |
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| 27421 | 3' | -52.8 | NC_005869.1 | + | 24483 | 1.08 | 0.000988 |
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Target: 5'- uACCUACGAGAGAGUUGACACGCUGCAu -3' miRNA: 3'- -UGGAUGCUCUCUCAACUGUGCGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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