Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27421 | 5' | -62.2 | NC_005869.1 | + | 17376 | 0.66 | 0.258184 |
Target: 5'- cCGCUCcagcGCCgcgGCCgCgCUgaGCuGCGGCg -3' miRNA: 3'- -GCGAGu---CGGa--CGG-G-GAgaCGuCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 9419 | 0.66 | 0.251627 |
Target: 5'- gCGCUCGauGaCCUcGCCCCgcaUGCGGC-GCa -3' miRNA: 3'- -GCGAGU--C-GGA-CGGGGag-ACGUCGcCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 17554 | 0.66 | 0.238924 |
Target: 5'- gGCgguggCAGCgCcGCCCgC-CcGCAGCGGCg -3' miRNA: 3'- gCGa----GUCG-GaCGGG-GaGaCGUCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 29273 | 0.66 | 0.238924 |
Target: 5'- gGcCUCAGUCUGCaagucacagcuCCC-CUGCAGUuccagGGCa -3' miRNA: 3'- gC-GAGUCGGACG-----------GGGaGACGUCG-----CCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 18762 | 0.66 | 0.238924 |
Target: 5'- cCGCccCGGCCcccgcgGCUCCUgUGCGuCGGCg -3' miRNA: 3'- -GCGa-GUCGGa-----CGGGGAgACGUcGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 12135 | 0.66 | 0.232776 |
Target: 5'- -cCUCGGUgCUGCCCaugcCUGC-GCGGCu -3' miRNA: 3'- gcGAGUCG-GACGGGga--GACGuCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 7258 | 0.66 | 0.226762 |
Target: 5'- gGUUCuG-CUGCCgCCgCUGCuGCGGCc -3' miRNA: 3'- gCGAGuCgGACGG-GGaGACGuCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 11704 | 0.67 | 0.22088 |
Target: 5'- aGCauggCGGCgaGCUCUgaagCUGgGGCGGCg -3' miRNA: 3'- gCGa---GUCGgaCGGGGa---GACgUCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 16556 | 0.67 | 0.22088 |
Target: 5'- cCGCUcCAGCacccacgccGCCCCUagccccGCGGCGGUc -3' miRNA: 3'- -GCGA-GUCGga-------CGGGGAga----CGUCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 27556 | 0.67 | 0.215128 |
Target: 5'- aCGCUgCAGaacuCCaGCuCCCUUccccGCAGCGGCg -3' miRNA: 3'- -GCGA-GUC----GGaCG-GGGAGa---CGUCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 13220 | 0.68 | 0.183264 |
Target: 5'- gGCUCGcGCC-GCUCCagcCUGgcCAGCGGCg -3' miRNA: 3'- gCGAGU-CGGaCGGGGa--GAC--GUCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 16658 | 0.68 | 0.178377 |
Target: 5'- aGCUCAGCgCgGCCgCggcgCUGgAGCGGg -3' miRNA: 3'- gCGAGUCG-GaCGGgGa---GACgUCGCCg -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 17823 | 0.68 | 0.164405 |
Target: 5'- gGUUCcGCgUGgCgCCUCggcGCAGCGGCg -3' miRNA: 3'- gCGAGuCGgACgG-GGAGa--CGUCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 20365 | 0.69 | 0.155644 |
Target: 5'- gGCggaGGCgacGCUCC-CUGCGGCGGCg -3' miRNA: 3'- gCGag-UCGga-CGGGGaGACGUCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 12275 | 0.69 | 0.143295 |
Target: 5'- cCGCUCggGGCC-GCacaCCUucCUGCAgGCGGCg -3' miRNA: 3'- -GCGAG--UCGGaCGg--GGA--GACGU-CGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 2575 | 0.7 | 0.114628 |
Target: 5'- gGCUCGGCgCUGCgCCCagcGCuuGCGGCa -3' miRNA: 3'- gCGAGUCG-GACG-GGGagaCGu-CGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 8511 | 0.71 | 0.108347 |
Target: 5'- uGC-CGGCCUccGCggCCUCgGCGGCGGCg -3' miRNA: 3'- gCGaGUCGGA--CGg-GGAGaCGUCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 24455 | 0.72 | 0.091389 |
Target: 5'- aGCUCAGCC-GCCgC-CUGgaAGCGGCc -3' miRNA: 3'- gCGAGUCGGaCGGgGaGACg-UCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 18113 | 0.72 | 0.086318 |
Target: 5'- cCGCUUGGCCUugcGCCgCUUCUuCAGCGGUu -3' miRNA: 3'- -GCGAGUCGGA---CGG-GGAGAcGUCGCCG- -5' |
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27421 | 5' | -62.2 | NC_005869.1 | + | 9806 | 0.75 | 0.049852 |
Target: 5'- gCGgUCGGCCaUGCCCCaggccuccugCUgGCAGCGGCc -3' miRNA: 3'- -GCgAGUCGG-ACGGGGa---------GA-CGUCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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