Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27423 | 3' | -55.8 | NC_005869.1 | + | 23712 | 0.67 | 0.458395 |
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Target: 5'- gGCCACCgcCggGCUCGGGgUCAccGGg -3' miRNA: 3'- gCGGUGGaaGagCGAGUCCgAGUa-CC- -5' |
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| 27423 | 3' | -55.8 | NC_005869.1 | + | 1505 | 0.68 | 0.408787 |
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Target: 5'- aGCCACC-UCUCuaugagaagcugGCUCAGGCUgGc-- -3' miRNA: 3'- gCGGUGGaAGAG------------CGAGUCCGAgUacc -5' |
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| 27423 | 3' | -55.8 | NC_005869.1 | + | 9394 | 0.72 | 0.246943 |
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Target: 5'- aGCCGCCagugCGCgUC-GGCUCGUGGg -3' miRNA: 3'- gCGGUGGaagaGCG-AGuCCGAGUACC- -5' |
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| 27423 | 3' | -55.8 | NC_005869.1 | + | 22105 | 0.73 | 0.209981 |
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Target: 5'- aG-CACCUUCUCGUgcgUCGGGUUCGUGc -3' miRNA: 3'- gCgGUGGAAGAGCG---AGUCCGAGUACc -5' |
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| 27423 | 3' | -55.8 | NC_005869.1 | + | 13196 | 0.75 | 0.150225 |
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Target: 5'- aCGcCCACCUauggcUCUCGCUgGGGCUCGc-- -3' miRNA: 3'- -GC-GGUGGA-----AGAGCGAgUCCGAGUacc -5' |
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| 27423 | 3' | -55.8 | NC_005869.1 | + | 31484 | 0.87 | 0.022357 |
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Target: 5'- aGCCACCggCUCGCUCA-GCUCGUGGc -3' miRNA: 3'- gCGGUGGaaGAGCGAGUcCGAGUACC- -5' |
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| 27423 | 3' | -55.8 | NC_005869.1 | + | 22748 | 1.11 | 0.00033 |
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Target: 5'- cCGCCACCUUCUCGCUCAGGCUCAUGGa -3' miRNA: 3'- -GCGGUGGAAGAGCGAGUCCGAGUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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