miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27423 5' -52.6 NC_005869.1 + 33088 0.66 0.715662
Target:  5'- aCACCugGAUGGg--GGGGCAGa---- -3'
miRNA:   3'- -GUGGugUUACCagaCCUCGUCcaagu -5'
27423 5' -52.6 NC_005869.1 + 17408 0.66 0.669421
Target:  5'- gCGCCACGAcGGcCUGGGGCcGGc--- -3'
miRNA:   3'- -GUGGUGUUaCCaGACCUCGuCCaagu -5'
27423 5' -52.6 NC_005869.1 + 20972 0.66 0.667085
Target:  5'- gGCCGCGAgggcggcuccucGGUCaGGGGCAGGg--- -3'
miRNA:   3'- gUGGUGUUa-----------CCAGaCCUCGUCCaagu -5'
27423 5' -52.6 NC_005869.1 + 4442 0.67 0.657728
Target:  5'- uGCCGCucUGGUCgcGGcGgGGGUUCAu -3'
miRNA:   3'- gUGGUGuuACCAGa-CCuCgUCCAAGU- -5'
27423 5' -52.6 NC_005869.1 + 30149 0.67 0.657728
Target:  5'- gCACCGCAG-GGUCgcucuccGGGGUGGGUg-- -3'
miRNA:   3'- -GUGGUGUUaCCAGa------CCUCGUCCAagu -5'
27423 5' -52.6 NC_005869.1 + 8350 0.67 0.646007
Target:  5'- aGCCuGCAGgaGGUCggGGGGCGGGcgCAg -3'
miRNA:   3'- gUGG-UGUUa-CCAGa-CCUCGUCCaaGU- -5'
27423 5' -52.6 NC_005869.1 + 17210 0.68 0.599103
Target:  5'- cCGCCACGGgcggcaguggGGUCUGuacGGGCAGGgcCAc -3'
miRNA:   3'- -GUGGUGUUa---------CCAGAC---CUCGUCCaaGU- -5'
27423 5' -52.6 NC_005869.1 + 15281 0.68 0.564218
Target:  5'- aGCCGCGggGG-CcGGGGCGGGggCGg -3'
miRNA:   3'- gUGGUGUuaCCaGaCCUCGUCCaaGU- -5'
27423 5' -52.6 NC_005869.1 + 25342 0.69 0.518616
Target:  5'- cCGCCAUGggGGUCUGGcAGCAGuGccUCGa -3'
miRNA:   3'- -GUGGUGUuaCCAGACC-UCGUC-Ca-AGU- -5'
27423 5' -52.6 NC_005869.1 + 17469 0.7 0.429358
Target:  5'- cCGCCGCGgggcuaggggcggcGUGGgugCUGGAGCGGGc--- -3'
miRNA:   3'- -GUGGUGU--------------UACCa--GACCUCGUCCaagu -5'
27423 5' -52.6 NC_005869.1 + 22784 1.08 0.000989
Target:  5'- gCACCACAAUGGUCUGGAGCAGGUUCAc -3'
miRNA:   3'- -GUGGUGUUACCAGACCUCGUCCAAGU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.