Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27426 | 5' | -55.8 | NC_005869.1 | + | 18090 | 0.66 | 0.515613 |
Target: 5'- aCCUCGUCGCUGUCGcucaaccCCCGc -3' miRNA: 3'- aGGAGCAGCGACAGUuuccgcaGGGU- -5' |
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27426 | 5' | -55.8 | NC_005869.1 | + | 6496 | 0.67 | 0.477243 |
Target: 5'- aCCUCGccgcggaggggcUCGUUGguccagCAGAGGCGgccgCCCu -3' miRNA: 3'- aGGAGC------------AGCGACa-----GUUUCCGCa---GGGu -5' |
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27426 | 5' | -55.8 | NC_005869.1 | + | 20576 | 0.72 | 0.226596 |
Target: 5'- cUCCUCGUCGUcuUCGAGGGC--CCCGg -3' miRNA: 3'- -AGGAGCAGCGacAGUUUCCGcaGGGU- -5' |
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27426 | 5' | -55.8 | NC_005869.1 | + | 9071 | 0.73 | 0.216961 |
Target: 5'- -gCUCGUCGCUGaccaccaggagguccUCGAgGGGCGUCUCGc -3' miRNA: 3'- agGAGCAGCGAC---------------AGUU-UCCGCAGGGU- -5' |
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27426 | 5' | -55.8 | NC_005869.1 | + | 9349 | 0.74 | 0.194399 |
Target: 5'- aCCUCGUCGgUGguuggauagucaggUAAGGGgGUCCCAc -3' miRNA: 3'- aGGAGCAGCgACa-------------GUUUCCgCAGGGU- -5' |
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27426 | 5' | -55.8 | NC_005869.1 | + | 21061 | 1.09 | 0.000473 |
Target: 5'- cUCCUCGUCGCUGUCAAAGGCGUCCCAg -3' miRNA: 3'- -AGGAGCAGCGACAGUUUCCGCAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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