Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27428 | 5' | -51.2 | NC_005869.1 | + | 9970 | 0.66 | 0.803332 |
Target: 5'- gGAUGGCCuGCGcAUgcgcugcagggaGUCUGAGAAGUc- -3' miRNA: 3'- gCUACUGG-CGC-UA------------CAGGCUCUUCAac -5' |
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27428 | 5' | -51.2 | NC_005869.1 | + | 20437 | 0.66 | 0.793126 |
Target: 5'- -cAUGGCCGUGGUGaguuggaCCGGGAGGg-- -3' miRNA: 3'- gcUACUGGCGCUACa------GGCUCUUCaac -5' |
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27428 | 5' | -51.2 | NC_005869.1 | + | 16976 | 0.67 | 0.772164 |
Target: 5'- aGgcGACCGCGGUGgCCGGGccGg-- -3' miRNA: 3'- gCuaCUGGCGCUACaGGCUCuuCaac -5' |
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27428 | 5' | -51.2 | NC_005869.1 | + | 19904 | 1.08 | 0.001877 |
Target: 5'- uCGAUGACCGCGAUGUCCGAGAAGUUGc -3' miRNA: 3'- -GCUACUGGCGCUACAGGCUCUUCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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