Results 21 - 35 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27434 | 5' | -62.2 | NC_005869.1 | + | 18244 | 0.75 | 0.051447 |
Target: 5'- gGCGCGAGaugGCCauggCCGAGCGCGUCGGu -3' miRNA: 3'- aCGCGCUC---UGGg---GGCUCGCGCGGUUc -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 18469 | 0.7 | 0.134738 |
Target: 5'- cGC-CGGGugCUCCGccuccGGCGCGCCAc- -3' miRNA: 3'- aCGcGCUCugGGGGC-----UCGCGCGGUuc -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 18647 | 0.69 | 0.150361 |
Target: 5'- cGCGCGcGGCCCuCCGAcgucgacgaugGCGgCGCCGGc -3' miRNA: 3'- aCGCGCuCUGGG-GGCU-----------CGC-GCGGUUc -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 18830 | 0.66 | 0.274779 |
Target: 5'- gGCgGCGGGcGCCCCCGuacauggAGCGCagGCCcAGu -3' miRNA: 3'- aCG-CGCUC-UGGGGGC-------UCGCG--CGGuUC- -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 20833 | 0.67 | 0.215273 |
Target: 5'- gGCGCGAG-CCCCagcgagagccauaggUGGGCGUgGCCGGc -3' miRNA: 3'- aCGCGCUCuGGGG---------------GCUCGCG-CGGUUc -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 22193 | 0.68 | 0.202098 |
Target: 5'- aGCGcCGAGACCCgucggCCGucaGCGCCAu- -3' miRNA: 3'- aCGC-GCUCUGGG-----GGCucgCGCGGUuc -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 22822 | 0.67 | 0.230369 |
Target: 5'- aGCcaGCG--GCUCUCGGGCGCGCCcAGg -3' miRNA: 3'- aCG--CGCucUGGGGGCUCGCGCGGuUC- -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 23398 | 0.71 | 0.10789 |
Target: 5'- aGCGCGucGCCCCCuccGCGC-CCAGGg -3' miRNA: 3'- aCGCGCucUGGGGGcu-CGCGcGGUUC- -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 24267 | 0.66 | 0.261905 |
Target: 5'- aGCGCGccggccgcaaAGACCucaCCCuccuGCGCGCCAu- -3' miRNA: 3'- aCGCGC----------UCUGG---GGGcu--CGCGCGGUuc -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 24423 | 0.69 | 0.146307 |
Target: 5'- cGCG-GAGGCCgCCGAG-GCGCCc-- -3' miRNA: 3'- aCGCgCUCUGGgGGCUCgCGCGGuuc -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 24551 | 0.7 | 0.127499 |
Target: 5'- cUGCGCGGGggcGCCUUCGAGCuGCGgccCCGGGa -3' miRNA: 3'- -ACGCGCUC---UGGGGGCUCG-CGC---GGUUC- -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 25800 | 0.71 | 0.104616 |
Target: 5'- gUGCGCuGGGACCCCCcgcccgaGGGCcCGCCGc- -3' miRNA: 3'- -ACGCG-CUCUGGGGG-------CUCGcGCGGUuc -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 27974 | 0.7 | 0.142354 |
Target: 5'- aGCGCGAGACgCCgGAGaCGCGgacuCCGGu -3' miRNA: 3'- aCGCGCUCUGgGGgCUC-GCGC----GGUUc -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 28444 | 0.79 | 0.028771 |
Target: 5'- aGUGCGAGACCCagugCGAGCGCuGCCGc- -3' miRNA: 3'- aCGCGCUCUGGGg---GCUCGCG-CGGUuc -5' |
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27434 | 5' | -62.2 | NC_005869.1 | + | 33691 | 0.68 | 0.204245 |
Target: 5'- aGCGcCGAGACgagugugagcgaacaCCgCGAGCGgGCCGGu -3' miRNA: 3'- aCGC-GCUCUG---------------GGgGCUCGCgCGGUUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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