Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27440 | 5' | -53.7 | NC_005869.1 | + | 27955 | 0.66 | 0.673355 |
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Target: 5'- gGACcaCAGAuGCAGCCgCAGCGcGAgacgCCg -3' miRNA: 3'- -CUGcaGUCU-UGUCGG-GUCGCaCUa---GG- -5' |
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| 27440 | 5' | -53.7 | NC_005869.1 | + | 11835 | 0.67 | 0.627371 |
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Target: 5'- aGCGUgAGGcaGCGGUCCGGgGUGcUCUg -3' miRNA: 3'- cUGCAgUCU--UGUCGGGUCgCACuAGG- -5' |
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| 27440 | 5' | -53.7 | NC_005869.1 | + | 18661 | 0.67 | 0.592888 |
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Target: 5'- cGACGUCGacGAugGCGGCgCCGGCGgcgggugGcgCCg -3' miRNA: 3'- -CUGCAGU--CU--UGUCG-GGUCGCa------CuaGG- -5' |
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| 27440 | 5' | -53.7 | NC_005869.1 | + | 8298 | 0.68 | 0.547461 |
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Target: 5'- gGACGa-AGGugGGCCCGGCGgcgcGggCCa -3' miRNA: 3'- -CUGCagUCUugUCGGGUCGCa---CuaGG- -5' |
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| 27440 | 5' | -53.7 | NC_005869.1 | + | 18796 | 0.68 | 0.536264 |
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Target: 5'- uGGCGUCGG-ACGaUCaCGGgGUGAUCCg -3' miRNA: 3'- -CUGCAGUCuUGUcGG-GUCgCACUAGG- -5' |
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| 27440 | 5' | -53.7 | NC_005869.1 | + | 2453 | 0.7 | 0.429855 |
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Target: 5'- uGugGUUuuugaucgGGAGCuaccGGCCCGGgGUGGUCUg -3' miRNA: 3'- -CugCAG--------UCUUG----UCGGGUCgCACUAGG- -5' |
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| 27440 | 5' | -53.7 | NC_005869.1 | + | 10860 | 0.7 | 0.409101 |
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Target: 5'- cGACGUgGGGuCGGCCCccaacaccuucucGGCGcGGUCCu -3' miRNA: 3'- -CUGCAgUCUuGUCGGG-------------UCGCaCUAGG- -5' |
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| 27440 | 5' | -53.7 | NC_005869.1 | + | 2364 | 0.71 | 0.381491 |
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Target: 5'- aGGCGgu-GAACcuGCCCAGCGUGAgUCg -3' miRNA: 3'- -CUGCaguCUUGu-CGGGUCGCACUaGG- -5' |
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| 27440 | 5' | -53.7 | NC_005869.1 | + | 806 | 1.12 | 0.000505 |
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Target: 5'- uGACGUCAGAACAGCCCAGCGUGAUCCu -3' miRNA: 3'- -CUGCAGUCUUGUCGGGUCGCACUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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