Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27441 | 5' | -55.1 | NC_005869.1 | + | 6492 | 0.72 | 0.259363 |
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Target: 5'- -gGCUACCuc-GCCgCGGAGgGGCUCg -3' miRNA: 3'- gaUGAUGGugaUGGgGCUUCgCCGAG- -5' |
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| 27441 | 5' | -55.1 | NC_005869.1 | + | 3702 | 0.73 | 0.226539 |
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Target: 5'- gCUGCggGCCACgGCCUCgGAGGCGGC-Cg -3' miRNA: 3'- -GAUGa-UGGUGaUGGGG-CUUCGCCGaG- -5' |
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| 27441 | 5' | -55.1 | NC_005869.1 | + | 18745 | 0.73 | 0.220412 |
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Target: 5'- -cGCUGCCGCcgccgcccccGCCCCGGcccccGCGGCUCc -3' miRNA: 3'- gaUGAUGGUGa---------UGGGGCUu----CGCCGAG- -5' |
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| 27441 | 5' | -55.1 | NC_005869.1 | + | 17599 | 0.73 | 0.214426 |
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Target: 5'- -cGCUGCCACcGCCaCCGGgguacGGCGGCg- -3' miRNA: 3'- gaUGAUGGUGaUGG-GGCU-----UCGCCGag -5' |
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| 27441 | 5' | -55.1 | NC_005869.1 | + | 12430 | 0.76 | 0.148603 |
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Target: 5'- -gGCUGCUGCUGCUCCu-GGUGGCUCc -3' miRNA: 3'- gaUGAUGGUGAUGGGGcuUCGCCGAG- -5' |
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| 27441 | 5' | -55.1 | NC_005869.1 | + | 14544 | 0.78 | 0.104737 |
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Target: 5'- -aGCUACCACgugggcgaggACCCCGAGGCGGg-- -3' miRNA: 3'- gaUGAUGGUGa---------UGGGGCUUCGCCgag -5' |
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| 27441 | 5' | -55.1 | NC_005869.1 | + | 3916 | 1.1 | 0.000415 |
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Target: 5'- gCUACUACCACUACCCCGAAGCGGCUCa -3' miRNA: 3'- -GAUGAUGGUGAUGGGGCUUCGCCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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