Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27442 | 3' | -59.1 | NC_005869.1 | + | 26264 | 0.67 | 0.334615 |
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Target: 5'- gGUGGUGGCGGCGGCGguggCUcGGCuGCu -3' miRNA: 3'- -CGCCACUGCUGCUGCag--GAcCCG-CGu -5' |
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| 27442 | 3' | -59.1 | NC_005869.1 | + | 26938 | 0.67 | 0.376877 |
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Target: 5'- uGCGGUuccuGAuuuUGAgGGCGUCCUGGcCGCc -3' miRNA: 3'- -CGCCA----CU---GCUgCUGCAGGACCcGCGu -5' |
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| 27442 | 3' | -59.1 | NC_005869.1 | + | 27980 | 0.69 | 0.267547 |
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Target: 5'- cGCGGUGGCGGCG--GUCgCUGuaGCGCGu -3' miRNA: 3'- -CGCCACUGCUGCugCAG-GACc-CGCGU- -5' |
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| 27442 | 3' | -59.1 | NC_005869.1 | + | 28653 | 0.71 | 0.206227 |
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Target: 5'- gGCGGUGGCaGCGGCG----GGGCGCGg -3' miRNA: 3'- -CGCCACUGcUGCUGCaggaCCCGCGU- -5' |
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| 27442 | 3' | -59.1 | NC_005869.1 | + | 28707 | 0.69 | 0.281452 |
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Target: 5'- aCGGuUGGCucaucguaGAUGugGUCCUcGGCGCAg -3' miRNA: 3'- cGCC-ACUG--------CUGCugCAGGAcCCGCGU- -5' |
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| 27442 | 3' | -59.1 | NC_005869.1 | + | 31497 | 0.68 | 0.325795 |
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Target: 5'- uGCGGcacuggcugaagcUGGCGGCGGaggCCaucgGGGCGCAg -3' miRNA: 3'- -CGCC-------------ACUGCUGCUgcaGGa---CCCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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