Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27442 | 3' | -59.1 | NC_005869.1 | + | 17584 | 0.71 | 0.194481 |
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Target: 5'- cGgGGUacGGCGGCGACG-CCgaggcgcucuggGGGCGCGa -3' miRNA: 3'- -CgCCA--CUGCUGCUGCaGGa-----------CCCGCGU- -5' |
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| 27442 | 3' | -59.1 | NC_005869.1 | + | 10611 | 0.74 | 0.109693 |
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Target: 5'- uGCGGcaGAUGAagccgccggcCGGCGcCCUGGGCGCGg -3' miRNA: 3'- -CGCCa-CUGCU----------GCUGCaGGACCCGCGU- -5' |
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| 27442 | 3' | -59.1 | NC_005869.1 | + | 13488 | 0.74 | 0.109384 |
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Target: 5'- gGCuGGUGGCGGCGggagaagcuggccACGggcaggcccaucUCCUGGGCGCGc -3' miRNA: 3'- -CG-CCACUGCUGC-------------UGC------------AGGACCCGCGU- -5' |
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| 27442 | 3' | -59.1 | NC_005869.1 | + | 18766 | 0.77 | 0.075775 |
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Target: 5'- gGUGGUGAgCGGCGGCGgg--GGGCGCAu -3' miRNA: 3'- -CGCCACU-GCUGCUGCaggaCCCGCGU- -5' |
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| 27442 | 3' | -59.1 | NC_005869.1 | + | 13361 | 0.82 | 0.029843 |
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Target: 5'- gGCGGccGGCGGCGGCG-CCUGGGUGCu -3' miRNA: 3'- -CGCCa-CUGCUGCUGCaGGACCCGCGu -5' |
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| 27442 | 3' | -59.1 | NC_005869.1 | + | 7052 | 1.1 | 0.000226 |
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Target: 5'- gGCGGUGACGACGACGUCCUGGGCGCAg -3' miRNA: 3'- -CGCCACUGCUGCUGCAGGACCCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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