Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27442 | 5' | -54.2 | NC_005869.1 | + | 20961 | 0.67 | 0.60302 |
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Target: 5'- -aUGGCCUccagggugGaGGCGGUGUUGUGGGCc- -3' miRNA: 3'- ccACCGGA--------C-CUGCUACAGCAUUCGca -5' |
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| 27442 | 5' | -54.2 | NC_005869.1 | + | 16264 | 0.67 | 0.60302 |
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Target: 5'- uGGUccGGCCgcgGGACGcggggGUCGgcgagGGGCGg -3' miRNA: 3'- -CCA--CCGGa--CCUGCua---CAGCa----UUCGCa -5' |
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| 27442 | 5' | -54.2 | NC_005869.1 | + | 22511 | 0.67 | 0.591642 |
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Target: 5'- cGUGGCggUGGGCGcgGaaGUAGGCGg -3' miRNA: 3'- cCACCGg-ACCUGCuaCagCAUUCGCa -5' |
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| 27442 | 5' | -54.2 | NC_005869.1 | + | 6680 | 0.68 | 0.524466 |
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Target: 5'- gGGcGGCCaGGGCGcgGUCGaAGGgGUu -3' miRNA: 3'- -CCaCCGGaCCUGCuaCAGCaUUCgCA- -5' |
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| 27442 | 5' | -54.2 | NC_005869.1 | + | 3465 | 0.68 | 0.51353 |
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Target: 5'- aGUGGCCgGGACGAaUGUgaaCGgcgGAGUGg -3' miRNA: 3'- cCACCGGaCCUGCU-ACA---GCa--UUCGCa -5' |
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| 27442 | 5' | -54.2 | NC_005869.1 | + | 18809 | 0.69 | 0.49195 |
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Target: 5'- cGGgGGCCgGGGCGggGgCGgcGGCGg -3' miRNA: 3'- -CCaCCGGaCCUGCuaCaGCauUCGCa -5' |
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| 27442 | 5' | -54.2 | NC_005869.1 | + | 17110 | 0.69 | 0.481317 |
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Target: 5'- cGGUGGCCgGGcCGGgcucCGUGAGCa- -3' miRNA: 3'- -CCACCGGaCCuGCUaca-GCAUUCGca -5' |
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| 27442 | 5' | -54.2 | NC_005869.1 | + | 10706 | 0.71 | 0.381941 |
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Target: 5'- gGGgagGGCCUGGcGCGGcUGUCGggcgcGGCGg -3' miRNA: 3'- -CCa--CCGGACC-UGCU-ACAGCau---UCGCa -5' |
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| 27442 | 5' | -54.2 | NC_005869.1 | + | 21410 | 0.71 | 0.337642 |
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Target: 5'- aGGUGGuaucCCUGGuagcCGAUGUUGUAgugGGCGa -3' miRNA: 3'- -CCACC----GGACCu---GCUACAGCAU---UCGCa -5' |
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| 27442 | 5' | -54.2 | NC_005869.1 | + | 7087 | 1.09 | 0.000752 |
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Target: 5'- gGGUGGCCUGGACGAUGUCGUAAGCGUc -3' miRNA: 3'- -CCACCGGACCUGCUACAGCAUUCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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