Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27446 | 3' | -54.6 | NC_005869.1 | + | 23650 | 0.66 | 0.593243 |
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Target: 5'- --aGGGCCUG-ACgCUGcUGCcGGACGg -3' miRNA: 3'- gcaCUCGGACaUG-GACuACGuCCUGC- -5' |
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| 27446 | 3' | -54.6 | NC_005869.1 | + | 7714 | 0.66 | 0.586406 |
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Target: 5'- uGUGGGCCagcagGUGCUgccagagggugucggUGAggcgggccUGCAGGGCGu -3' miRNA: 3'- gCACUCGGa----CAUGG---------------ACU--------ACGUCCUGC- -5' |
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| 27446 | 3' | -54.6 | NC_005869.1 | + | 7522 | 0.67 | 0.525777 |
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Target: 5'- gGUGGGCgaGcACCUGcgcgGCcAGGACGa -3' miRNA: 3'- gCACUCGgaCaUGGACua--CG-UCCUGC- -5' |
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| 27446 | 3' | -54.6 | NC_005869.1 | + | 22805 | 0.71 | 0.321361 |
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Target: 5'- uGUGGGCCgc--CCUGAUGCAG-ACGc -3' miRNA: 3'- gCACUCGGacauGGACUACGUCcUGC- -5' |
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| 27446 | 3' | -54.6 | NC_005869.1 | + | 14701 | 0.85 | 0.035614 |
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Target: 5'- --gGAGCCUGccggACCUGAUGCAGGACc -3' miRNA: 3'- gcaCUCGGACa---UGGACUACGUCCUGc -5' |
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| 27446 | 3' | -54.6 | NC_005869.1 | + | 12718 | 1.1 | 0.000506 |
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Target: 5'- cCGUGAGCCUGUACCUGAUGCAGGACGg -3' miRNA: 3'- -GCACUCGGACAUGGACUACGUCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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