Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2745 | 5' | -55.6 | NC_001491.2 | + | 120150 | 0.66 | 0.911572 |
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Target: 5'- gGGCCGGCGCgUGUGUguauagCGucacAUCGugGc -3' miRNA: 3'- aCCGGUCGCGgACAUA------GC----UAGCugUa -5' |
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| 2745 | 5' | -55.6 | NC_001491.2 | + | 17919 | 0.66 | 0.911572 |
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Target: 5'- aGGCCGGCGCCauUGUUGA-CGGa-- -3' miRNA: 3'- aCCGGUCGCGGacAUAGCUaGCUgua -5' |
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| 2745 | 5' | -55.6 | NC_001491.2 | + | 9517 | 0.66 | 0.898959 |
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Target: 5'- gGGCCGuGCGCCUGg--CGcuaccuagguacGUCGACc- -3' miRNA: 3'- aCCGGU-CGCGGACauaGC------------UAGCUGua -5' |
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| 2745 | 5' | -55.6 | NC_001491.2 | + | 143884 | 0.67 | 0.855518 |
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Target: 5'- gUGGCCAGC-CCacacUAUCGAUUGugAUu -3' miRNA: 3'- -ACCGGUCGcGGac--AUAGCUAGCugUA- -5' |
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| 2745 | 5' | -55.6 | NC_001491.2 | + | 145674 | 0.68 | 0.804611 |
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Target: 5'- cGGCCGGUGCCUGgGUCugGGUCuGGgGUa -3' miRNA: 3'- aCCGGUCGCGGACaUAG--CUAG-CUgUA- -5' |
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| 2745 | 5' | -55.6 | NC_001491.2 | + | 147713 | 0.75 | 0.409622 |
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Target: 5'- gUGGCCAGgGCCUGgggAUCGAagGugAg -3' miRNA: 3'- -ACCGGUCgCGGACa--UAGCUagCugUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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