Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27452 | 5' | -59.6 | NC_005869.1 | + | 16522 | 0.66 | 0.317198 |
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Target: 5'- -aGGCgGUgggCUCGUCggcgggCGCCCGGCGc -3' miRNA: 3'- uaCCGgUAg--GAGCAGa-----GCGGGUCGUu -5' |
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| 27452 | 5' | -59.6 | NC_005869.1 | + | 20883 | 0.66 | 0.317198 |
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Target: 5'- uUGGCCccGUCCgcgcggagGUCgUUGCCCAGCGu -3' miRNA: 3'- uACCGG--UAGGag------CAG-AGCGGGUCGUu -5' |
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| 27452 | 5' | -59.6 | NC_005869.1 | + | 33650 | 0.66 | 0.278879 |
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Target: 5'- -cGGUUucUCCUCGUCUCGCUcuCAGUg- -3' miRNA: 3'- uaCCGGu-AGGAGCAGAGCGG--GUCGuu -5' |
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| 27452 | 5' | -59.6 | NC_005869.1 | + | 15614 | 0.67 | 0.25095 |
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Target: 5'- -cGGCCAUCCUgGgggugCGCCgCAGCc- -3' miRNA: 3'- uaCCGGUAGGAgCaga--GCGG-GUCGuu -5' |
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| 27452 | 5' | -59.6 | NC_005869.1 | + | 33620 | 0.68 | 0.225368 |
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Target: 5'- gAUGGCCGgugaCUCcUCUCGCCCcGCu- -3' miRNA: 3'- -UACCGGUag--GAGcAGAGCGGGuCGuu -5' |
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| 27452 | 5' | -59.6 | NC_005869.1 | + | 17931 | 0.68 | 0.219328 |
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Target: 5'- -gGGCCAUCCcCGgcaUCGCCUcgguGGCGAu -3' miRNA: 3'- uaCCGGUAGGaGCag-AGCGGG----UCGUU- -5' |
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| 27452 | 5' | -59.6 | NC_005869.1 | + | 7642 | 0.72 | 0.104982 |
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Target: 5'- -aGGCCAgggggUCCUCGgggUCGUCCGGCAGg -3' miRNA: 3'- uaCCGGU-----AGGAGCag-AGCGGGUCGUU- -5' |
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| 27452 | 5' | -59.6 | NC_005869.1 | + | 12638 | 0.72 | 0.104982 |
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Target: 5'- -cGcGUCGUCCUCGUCgcccaaCGCCCGGCu- -3' miRNA: 3'- uaC-CGGUAGGAGCAGa-----GCGGGUCGuu -5' |
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| 27452 | 5' | -59.6 | NC_005869.1 | + | 15153 | 1.03 | 0.000366 |
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Target: 5'- cAUGGCCAUCCUCGUCUCGCCCAGCAAc -3' miRNA: 3'- -UACCGGUAGGAGCAGAGCGGGUCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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