Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27455 | 5' | -59.3 | NC_005869.1 | + | 15477 | 0.66 | 0.344567 |
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Target: 5'- aGCCCggcGCCG-CCGCGCg--CGGCGc- -3' miRNA: 3'- aCGGG---UGGCaGGCGUGacaGUCGCac -5' |
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| 27455 | 5' | -59.3 | NC_005869.1 | + | 16722 | 0.66 | 0.344567 |
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Target: 5'- cGCCCgcuccagcGCCGcggCCGCGCUGagcugCGGCGc- -3' miRNA: 3'- aCGGG--------UGGCa--GGCGUGACa----GUCGCac -5' |
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| 27455 | 5' | -59.3 | NC_005869.1 | + | 9848 | 0.67 | 0.296905 |
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Target: 5'- gGCCgACCGcaUCCGC-CUG-CAGCGc- -3' miRNA: 3'- aCGGgUGGC--AGGCGuGACaGUCGCac -5' |
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| 27455 | 5' | -59.3 | NC_005869.1 | + | 5299 | 0.68 | 0.247961 |
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Target: 5'- cGCCCACCGccucguUCCGUACUcacgCAGCa-- -3' miRNA: 3'- aCGGGUGGC------AGGCGUGAca--GUCGcac -5' |
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| 27455 | 5' | -59.3 | NC_005869.1 | + | 20883 | 0.72 | 0.135773 |
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Target: 5'- uUGgCC-CCGUCCGCGCggagGUCguugcccAGCGUGg -3' miRNA: 3'- -ACgGGuGGCAGGCGUGa---CAG-------UCGCAC- -5' |
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| 27455 | 5' | -59.3 | NC_005869.1 | + | 17242 | 1.09 | 0.000211 |
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Target: 5'- gUGCCCACCGUCCGCACUGUCAGCGUGg -3' miRNA: 3'- -ACGGGUGGCAGGCGUGACAGUCGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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