Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27457 | 3' | -52.4 | NC_005869.1 | + | 18615 | 0.66 | 0.766629 |
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Target: 5'- -cACcgGGUCC-CCGGUgacggCCACGgaGGa -3' miRNA: 3'- guUGuaCUAGGaGGUCA-----GGUGCgaCC- -5' |
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| 27457 | 3' | -52.4 | NC_005869.1 | + | 28377 | 0.66 | 0.755845 |
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Target: 5'- aGAUGUGGUCCUCCGaggccGUCC-UGC-GGa -3' miRNA: 3'- gUUGUACUAGGAGGU-----CAGGuGCGaCC- -5' |
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| 27457 | 3' | -52.4 | NC_005869.1 | + | 23974 | 0.66 | 0.711439 |
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Target: 5'- ---gGUGGUCaaCUGGUCCGUGCUGGg -3' miRNA: 3'- guugUACUAGgaGGUCAGGUGCGACC- -5' |
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| 27457 | 3' | -52.4 | NC_005869.1 | + | 21737 | 0.68 | 0.619288 |
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Target: 5'- uCGACAUGA-CCUUCgaGGUCgACGCcaUGGa -3' miRNA: 3'- -GUUGUACUaGGAGG--UCAGgUGCG--ACC- -5' |
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| 27457 | 3' | -52.4 | NC_005869.1 | + | 7375 | 0.69 | 0.584609 |
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Target: 5'- --cCAUGAcgcccUCCUcCCAGUCCGCGUa-- -3' miRNA: 3'- guuGUACU-----AGGA-GGUCAGGUGCGacc -5' |
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| 27457 | 3' | -52.4 | NC_005869.1 | + | 821 | 0.74 | 0.296732 |
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Target: 5'- cCAGCGUGAUCCUuuuuaaugugCUAGUCUACGUgcccacUGGg -3' miRNA: 3'- -GUUGUACUAGGA----------GGUCAGGUGCG------ACC- -5' |
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| 27457 | 3' | -52.4 | NC_005869.1 | + | 20796 | 1.11 | 0.000751 |
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Target: 5'- uCAACAUGAUCCUCCAGUCCACGCUGGg -3' miRNA: 3'- -GUUGUACUAGGAGGUCAGGUGCGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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