Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27459 | 3' | -59 | NC_005869.1 | + | 7741 | 0.66 | 0.389226 |
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Target: 5'- gACCUcgccgagcuggcgcaGGAGC-GGGGCCCCgGCcUGc -3' miRNA: 3'- gUGGA---------------CCUUGaCCCCGGGGgUGaACu -5' |
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| 27459 | 3' | -59 | NC_005869.1 | + | 12026 | 0.66 | 0.358895 |
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Target: 5'- gCugCUGGAGC-GGGuCUCCCGCUa-- -3' miRNA: 3'- -GugGACCUUGaCCCcGGGGGUGAacu -5' |
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| 27459 | 3' | -59 | NC_005869.1 | + | 18883 | 0.67 | 0.294249 |
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Target: 5'- aCACCauugUGGGACUGGGuGUCCgCACa--- -3' miRNA: 3'- -GUGG----ACCUUGACCC-CGGGgGUGaacu -5' |
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| 27459 | 3' | -59 | NC_005869.1 | + | 24051 | 0.7 | 0.209129 |
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Target: 5'- aGCCaGuGAGCUGGGGUCCuCCGCg--- -3' miRNA: 3'- gUGGaC-CUUGACCCCGGG-GGUGaacu -5' |
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| 27459 | 3' | -59 | NC_005869.1 | + | 5642 | 0.71 | 0.177545 |
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Target: 5'- -uUCUGGGgaucgaagACgagGGGGCCCCCguuccGCUUGAg -3' miRNA: 3'- guGGACCU--------UGa--CCCCGGGGG-----UGAACU- -5' |
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| 27459 | 3' | -59 | NC_005869.1 | + | 12358 | 0.71 | 0.158911 |
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Target: 5'- gGCCccugugGGGGCUGcaGGCCCCCGCUgggGAg -3' miRNA: 3'- gUGGa-----CCUUGACc-CCGGGGGUGAa--CU- -5' |
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| 27459 | 3' | -59 | NC_005869.1 | + | 5719 | 0.74 | 0.100885 |
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Target: 5'- cCGCCUcaagcGGAACgGGGGCCCCCucguCUUcGAu -3' miRNA: 3'- -GUGGA-----CCUUGaCCCCGGGGGu---GAA-CU- -5' |
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| 27459 | 3' | -59 | NC_005869.1 | + | 22698 | 1.08 | 0.000234 |
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Target: 5'- gCACCUGGAACUGGGGCCCCCACUUGAa -3' miRNA: 3'- -GUGGACCUUGACCCCGGGGGUGAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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