miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27462 3' -58.8 NC_005869.1 + 7249 0.66 0.340866
Target:  5'- --aCGUGGgcugguUCUGCUGCCGCcgCUGCUg -3'
miRNA:   3'- uagGUACC------GGGCGACGGUGaaGACGA- -5'
27462 3' -58.8 NC_005869.1 + 7844 0.66 0.315994
Target:  5'- -gCgGUGGCUCgGCUGCUGCUggUGCUg -3'
miRNA:   3'- uaGgUACCGGG-CGACGGUGAagACGA- -5'
27462 3' -58.8 NC_005869.1 + 32102 0.67 0.308013
Target:  5'- cAUCCcgGcCCCGCUGCCACU---GCc -3'
miRNA:   3'- -UAGGuaCcGGGCGACGGUGAagaCGa -5'
27462 3' -58.8 NC_005869.1 + 30859 0.67 0.277635
Target:  5'- -aCCGgggGGCCCGCagcgcgucUGUCACUuucaUCUGCc -3'
miRNA:   3'- uaGGUa--CCGGGCG--------ACGGUGA----AGACGa -5'
27462 3' -58.8 NC_005869.1 + 10342 0.67 0.277635
Target:  5'- -gCgGUGGCCCG--GCCGCUgCUGCa -3'
miRNA:   3'- uaGgUACCGGGCgaCGGUGAaGACGa -5'
27462 3' -58.8 NC_005869.1 + 9897 0.73 0.104002
Target:  5'- uUCgGUGGgcuCCUGCUGCCGCUgCUGCa -3'
miRNA:   3'- uAGgUACC---GGGCGACGGUGAaGACGa -5'
27462 3' -58.8 NC_005869.1 + 23657 1.05 0.000332
Target:  5'- gAUCCAUGGCCCGCUGCCACUUCUGCUc -3'
miRNA:   3'- -UAGGUACCGGGCGACGGUGAAGACGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.