Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27465 | 5' | -57.8 | NC_005869.1 | + | 14536 | 0.66 | 0.424761 |
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Target: 5'- aGGGUcGCAgcUACCacgugggcgaGGACCCCGAGGc -3' miRNA: 3'- aCCUA-CGU--GUGGag--------CCUGGGGUUCCa -5' |
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| 27465 | 5' | -57.8 | NC_005869.1 | + | 31929 | 0.67 | 0.375938 |
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Target: 5'- aGGAUGUACGCCaggGGACCacaaucuccaaaCAGGGa -3' miRNA: 3'- aCCUACGUGUGGag-CCUGGg-----------GUUCCa -5' |
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| 27465 | 5' | -57.8 | NC_005869.1 | + | 32454 | 0.67 | 0.359943 |
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Target: 5'- cUGGGaGUACACC-CGGACCC--GGGc -3' miRNA: 3'- -ACCUaCGUGUGGaGCCUGGGguUCCa -5' |
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| 27465 | 5' | -57.8 | NC_005869.1 | + | 9011 | 0.67 | 0.342735 |
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Target: 5'- cGGucuUGCGCcgcuccuccaggACCUUGGGgUCCGAGGUg -3' miRNA: 3'- aCCu--ACGUG------------UGGAGCCUgGGGUUCCA- -5' |
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| 27465 | 5' | -57.8 | NC_005869.1 | + | 2585 | 0.68 | 0.318052 |
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Target: 5'- gGGAUGCuacuuuuuCGCCUgcuacaaggcgcUGGACCaCCGGGGg -3' miRNA: 3'- aCCUACGu-------GUGGA------------GCCUGG-GGUUCCa -5' |
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| 27465 | 5' | -57.8 | NC_005869.1 | + | 22426 | 0.68 | 0.318052 |
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Target: 5'- gGGAcUGCcaccucacGCGCCgCGGuCCCCGGGGa -3' miRNA: 3'- aCCU-ACG--------UGUGGaGCCuGGGGUUCCa -5' |
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| 27465 | 5' | -57.8 | NC_005869.1 | + | 16167 | 0.72 | 0.182185 |
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Target: 5'- aGGAUGC-CACCccucaUCGGcCCCCGGcGGUa -3' miRNA: 3'- aCCUACGuGUGG-----AGCCuGGGGUU-CCA- -5' |
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| 27465 | 5' | -57.8 | NC_005869.1 | + | 25001 | 1.06 | 0.000425 |
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Target: 5'- cUGGAUGCACACCUCGGACCCCAAGGUc -3' miRNA: 3'- -ACCUACGUGUGGAGCCUGGGGUUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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