Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27469 | 3' | -49.9 | NC_005869.1 | + | 17218 | 0.66 | 0.843614 |
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Target: 5'- gGGGUGCAGaCCAUCGACgucACCg----- -3' miRNA: 3'- -UCCACGUUcGGUAGUUG---UGGaaacuc -5' |
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| 27469 | 3' | -49.9 | NC_005869.1 | + | 16645 | 0.67 | 0.782455 |
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Target: 5'- gGGGgGCAccaccGCCGUCAgccGCACCUgccUGGGu -3' miRNA: 3'- -UCCaCGUu----CGGUAGU---UGUGGAa--ACUC- -5' |
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| 27469 | 3' | -49.9 | NC_005869.1 | + | 10757 | 0.67 | 0.760435 |
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Target: 5'- cGGGUGCAgcucaagcgagaGGCCAUgGAgGCCUaUGuGc -3' miRNA: 3'- -UCCACGU------------UCGGUAgUUgUGGAaACuC- -5' |
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| 27469 | 3' | -49.9 | NC_005869.1 | + | 24815 | 0.68 | 0.753701 |
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Target: 5'- gAGGUGCGgcaggcgguggccgaGGCCAUCGcCGCCcuggaaGAGg -3' miRNA: 3'- -UCCACGU---------------UCGGUAGUuGUGGaaa---CUC- -5' |
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| 27469 | 3' | -49.9 | NC_005869.1 | + | 6020 | 0.68 | 0.702906 |
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Target: 5'- gGGGUGCGuGGCCGUCAGUugCUUcGcAGg -3' miRNA: 3'- -UCCACGU-UCGGUAGUUGugGAAaC-UC- -5' |
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| 27469 | 3' | -49.9 | NC_005869.1 | + | 26183 | 0.69 | 0.663696 |
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Target: 5'- cAGGUGguGGCCGUCcagagcccgccugcAGCGCUg--GAGa -3' miRNA: 3'- -UCCACguUCGGUAG--------------UUGUGGaaaCUC- -5' |
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| 27469 | 3' | -49.9 | NC_005869.1 | + | 25812 | 1.09 | 0.001658 |
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Target: 5'- gAGGUGCAAGCCAUCAACACCUUUGAGc -3' miRNA: 3'- -UCCACGUUCGGUAGUUGUGGAAACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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