Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2747 | 5' | -47.7 | NC_001491.2 | + | 116633 | 0.66 | 0.99977 |
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Target: 5'- --cUgCGUGCUGGCcugCCAGGcCGUCu -3' miRNA: 3'- caaAgGUACGAUUGa--GGUCUuGCAGc -5' |
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| 2747 | 5' | -47.7 | NC_001491.2 | + | 138501 | 0.66 | 0.999532 |
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Target: 5'- --gUCCGcgcUGCUGGCaUCGGcGGCGUCGg -3' miRNA: 3'- caaAGGU---ACGAUUGaGGUC-UUGCAGC- -5' |
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| 2747 | 5' | -47.7 | NC_001491.2 | + | 26901 | 0.66 | 0.999415 |
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Target: 5'- --cUCCAUcaaGCgcGCUCCAGcGCGUgGa -3' miRNA: 3'- caaAGGUA---CGauUGAGGUCuUGCAgC- -5' |
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| 2747 | 5' | -47.7 | NC_001491.2 | + | 94396 | 0.67 | 0.999274 |
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Target: 5'- --cUCCGUGCUuuuuaucACUCUAGugacGGCGUCu -3' miRNA: 3'- caaAGGUACGAu------UGAGGUC----UUGCAGc -5' |
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| 2747 | 5' | -47.7 | NC_001491.2 | + | 123114 | 0.68 | 0.998039 |
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Target: 5'- ---gCCcgGCgccGCUCCGGAGCG-CGu -3' miRNA: 3'- caaaGGuaCGau-UGAGGUCUUGCaGC- -5' |
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| 2747 | 5' | -47.7 | NC_001491.2 | + | 146023 | 0.68 | 0.997195 |
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Target: 5'- ---aCCAUGCgga--CCAGAGCGUUGu -3' miRNA: 3'- caaaGGUACGauugaGGUCUUGCAGC- -5' |
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| 2747 | 5' | -47.7 | NC_001491.2 | + | 144572 | 0.7 | 0.988927 |
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Target: 5'- ----gCGUGCUAGCUCCGGcuucGGgGUCGa -3' miRNA: 3'- caaagGUACGAUUGAGGUC----UUgCAGC- -5' |
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| 2747 | 5' | -47.7 | NC_001491.2 | + | 57860 | 0.75 | 0.904596 |
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Target: 5'- --cUCCAUGCUGACUCUGGccAUGUCc -3' miRNA: 3'- caaAGGUACGAUUGAGGUCu-UGCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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