miRNA: 3'- -GAGGau------GUGGU-----------UGUCGUCGUCGUCGU- -5'
miRNA: 3'- -GAGGA--UGU-GGUUGuCGUCGUCGUCGu -5'
miRNA: 3'- -GAGGAUGUGGUUGUCGUCGUCGUCGU- -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27470 | 3' | -54.6 | NC_005869.1 | + | 24109 | 0.81 | 0.053565 |
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Target: 5'- cCUCCgcccacguCACCGccaccuucacuGCAGCAGCGGCAGCAg -3' miRNA: 3'- -GAGGau------GUGGU-----------UGUCGUCGUCGUCGU- -5' |
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| 27470 | 3' | -54.6 | NC_005869.1 | + | 23681 | 0.83 | 0.043105 |
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Target: 5'- gCUCCUgaGCAuCCAGCuGCAGCAGCGGCc -3' miRNA: 3'- -GAGGA--UGU-GGUUGuCGUCGUCGUCGu -5' |
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| 27470 | 3' | -54.6 | NC_005869.1 | + | 26470 | 1.09 | 0.000451 |
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Target: 5'- cCUCCUACACCAACAGCAGCAGCAGCAa -3' miRNA: 3'- -GAGGAUGUGGUUGUCGUCGUCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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