miRNA: 3'- gagGAUGUGGuuGUCGUCGUCGUCgu -5'
miRNA: 3'- gAGGa-UGUgGUUGUCGUcGUCGUCGU- -5'
miRNA: 3'- gAGgaUGUGGuUGUC-GUCGUCGUCGu -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27470 | 3' | -54.6 | NC_005869.1 | + | 26998 | 0.68 | 0.419554 |
|
Target: 5'- --aCUGCGCU--CGGCAGCGGCAGa- -3' miRNA: 3'- gagGAUGUGGuuGUCGUCGUCGUCgu -5' |
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| 27470 | 3' | -54.6 | NC_005869.1 | + | 27578 | 0.71 | 0.30212 |
|
Target: 5'- uUCCccGCAgCGGCGGCAuCGGCAGCGa -3' miRNA: 3'- gAGGa-UGUgGUUGUCGUcGUCGUCGU- -5' |
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| 27470 | 3' | -54.6 | NC_005869.1 | + | 33563 | 0.67 | 0.460858 |
|
Target: 5'- gUCa-GCACC-GCGGcCAGCAGCAGUc -3' miRNA: 3'- gAGgaUGUGGuUGUC-GUCGUCGUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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