Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27474 | 3' | -48.6 | NC_005869.1 | + | 9377 | 0.66 | 0.929133 |
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Target: 5'- aGGCCGGggaCggCGCCGGCgcaaggGCAGCc -3' miRNA: 3'- gCUGGUCaa-GuaGCGGUUGa-----UGUUGu -5' |
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| 27474 | 3' | -48.6 | NC_005869.1 | + | 23216 | 0.67 | 0.894592 |
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Target: 5'- uGGCCccgUCGUUGCgCAGCUGCAcCAc -3' miRNA: 3'- gCUGGucaAGUAGCG-GUUGAUGUuGU- -5' |
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| 27474 | 3' | -48.6 | NC_005869.1 | + | 24112 | 0.67 | 0.894592 |
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Target: 5'- cCGcCCAcgUCAcCGCCAccuucACUGCAGCAg -3' miRNA: 3'- -GCuGGUcaAGUaGCGGU-----UGAUGUUGU- -5' |
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| 27474 | 3' | -48.6 | NC_005869.1 | + | 7253 | 0.68 | 0.833386 |
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Target: 5'- gGGCUGGUUCugcugcCGCCGcuGCUGCGGCc -3' miRNA: 3'- gCUGGUCAAGua----GCGGU--UGAUGUUGu -5' |
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| 27474 | 3' | -48.6 | NC_005869.1 | + | 21318 | 0.72 | 0.590935 |
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Target: 5'- aGACUGGUUCcuggugcagaugcUCGCCcACUACAACAu -3' miRNA: 3'- gCUGGUCAAGu------------AGCGGuUGAUGUUGU- -5' |
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| 27474 | 3' | -48.6 | NC_005869.1 | + | 27674 | 1.1 | 0.002362 |
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Target: 5'- cCGACCAGUUCAUCGCCAACUACAACAu -3' miRNA: 3'- -GCUGGUCAAGUAGCGGUUGAUGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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