Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27474 | 5' | -51.5 | NC_005869.1 | + | 6215 | 0.66 | 0.753639 |
Target: 5'- uGGCUUcgGUGguGCGGGCguccaucUGGCUGGCGa -3' miRNA: 3'- -CUGAGa-CAU--CGUCCGau-----ACUGACUGC- -5' |
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27474 | 5' | -51.5 | NC_005869.1 | + | 17637 | 0.68 | 0.624901 |
Target: 5'- gGGCUCgGUGagcggcuGCAGGCUggGugUGugGu -3' miRNA: 3'- -CUGAGaCAU-------CGUCCGAuaCugACugC- -5' |
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27474 | 5' | -51.5 | NC_005869.1 | + | 2611 | 0.73 | 0.33835 |
Target: 5'- aGGCgCUGgaccaccgggGGC-GGCUGUGGCUGACGg -3' miRNA: 3'- -CUGaGACa---------UCGuCCGAUACUGACUGC- -5' |
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27474 | 5' | -51.5 | NC_005869.1 | + | 27708 | 1.1 | 0.00098 |
Target: 5'- gGACUCUGUAGCAGGCUAUGACUGACGg -3' miRNA: 3'- -CUGAGACAUCGUCCGAUACUGACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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