Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27478 | 3' | -57.5 | NC_005869.1 | + | 25046 | 0.66 | 0.393297 |
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Target: 5'- ---aGGCGCCCUucaacCGCCUgguggcgcucaaccUGGC-CCAGg -3' miRNA: 3'- guuaCCGCGGGA-----GUGGA--------------ACCGaGGUU- -5' |
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| 27478 | 3' | -57.5 | NC_005869.1 | + | 29084 | 0.66 | 0.386702 |
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Target: 5'- ---aGG-GCCCUCACCcUGGcCUUCGGg -3' miRNA: 3'- guuaCCgCGGGAGUGGaACC-GAGGUU- -5' |
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| 27478 | 3' | -57.5 | NC_005869.1 | + | 17903 | 0.67 | 0.35928 |
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Target: 5'- --cUGGUGCCCaUCAUCgcGGCaUCCAu -3' miRNA: 3'- guuACCGCGGG-AGUGGaaCCG-AGGUu -5' |
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| 27478 | 3' | -57.5 | NC_005869.1 | + | 7175 | 0.67 | 0.35928 |
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Target: 5'- ---gGGCGUCCUgGCCgccgcGGCUCUg- -3' miRNA: 3'- guuaCCGCGGGAgUGGaa---CCGAGGuu -5' |
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| 27478 | 3' | -57.5 | NC_005869.1 | + | 18791 | 0.67 | 0.341768 |
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Target: 5'- ---cGGCGUCCg-GCCgcGGCUCCGg -3' miRNA: 3'- guuaCCGCGGGagUGGaaCCGAGGUu -5' |
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| 27478 | 3' | -57.5 | NC_005869.1 | + | 6488 | 0.68 | 0.292992 |
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Target: 5'- aGGUGGCuaCCUCGCCgcggaggGGCUCg-- -3' miRNA: 3'- gUUACCGcgGGAGUGGaa-----CCGAGguu -5' |
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| 27478 | 3' | -57.5 | NC_005869.1 | + | 18191 | 0.69 | 0.270717 |
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Target: 5'- ---aGGCGCCCcguaCACCUcgGGCgCCAAc -3' miRNA: 3'- guuaCCGCGGGa---GUGGAa-CCGaGGUU- -5' |
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| 27478 | 3' | -57.5 | NC_005869.1 | + | 17403 | 0.69 | 0.253894 |
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Target: 5'- gCGGUGGUGCCCccCACCccgcgcgccccggUGGUUCCGGu -3' miRNA: 3'- -GUUACCGCGGGa-GUGGa------------ACCGAGGUU- -5' |
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| 27478 | 3' | -57.5 | NC_005869.1 | + | 30372 | 1.06 | 0.000407 |
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Target: 5'- aCAAUGGCGCCCUCACCUUGGCUCCAAc -3' miRNA: 3'- -GUUACCGCGGGAGUGGAACCGAGGUU- -5' |
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| 27478 | 3' | -57.5 | NC_005869.1 | + | 29649 | 1.06 | 0.000407 |
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Target: 5'- aCAAUGGCGCCCUCACCUUGGCUCCAAc -3' miRNA: 3'- -GUUACCGCGGGAGUGGAACCGAGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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