miRNA: 3'- gUCGaaGUCU----------UC-GCGGUAUUGU-GGGG- -5'
miRNA: 3'- gUCGaa-GUCuUCGCGGUaUUguGGGG- -5'
miRNA: 3'- -GUCGA------AGUCUUCGcGGUa-UUG------UGGGG- -5'
miRNA: 3'- gUCGAa---GUCU---UCGcGGUAUUGuGGGG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2748 | 3' | -51.8 | NC_001491.2 | + | 146214 | 0.68 | 0.941133 |
|
Target: 5'- gGGCc-CAGAgcgcguucccGGcCGCCAUGGCGuCCCCg -3' miRNA: 3'- gUCGaaGUCU----------UC-GCGGUAUUGU-GGGG- -5' |
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| 2748 | 3' | -51.8 | NC_001491.2 | + | 147973 | 0.66 | 0.981764 |
|
Target: 5'- gGGCcgcCAGcAGCGCCGaGAguCCCCc -3' miRNA: 3'- gUCGaa-GUCuUCGCGGUaUUguGGGG- -5' |
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| 2748 | 3' | -51.8 | NC_001491.2 | + | 148689 | 0.68 | 0.954433 |
|
Target: 5'- gCAGCUcggggaUCAGAAGCuCCGagGGCuccacgGCCCCa -3' miRNA: 3'- -GUCGA------AGUCUUCGcGGUa-UUG------UGGGG- -5' |
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| 2748 | 3' | -51.8 | NC_001491.2 | + | 148868 | 0.67 | 0.962071 |
|
Target: 5'- gGGCUccggCAGGcacAGCuCCGUGGC-CCCCa -3' miRNA: 3'- gUCGAa---GUCU---UCGcGGUAUUGuGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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