miRNA: 3'- -CGUA-GUC-CCUCGCGUaGUggUGG-ACG- -5'
miRNA: 3'- -CGUaGUCCC---UCgCGUaGUGGUGGa---CG- -5'
miRNA: 3'- -CGUa---GUCCCU----CGCGUagUGGUGGACg -5'
miRNA: 3'- cGUAGUCCCu------------CGCGUaGu-GGUGGACg -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27480 | 5' | -55.5 | NC_005869.1 | + | 8969 | 0.66 | 0.569007 |
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Target: 5'- uGCAUgCAGuGGAGUGCAcCGugACCaUGCa -3' miRNA: 3'- -CGUA-GUC-CCUCGCGUaGUggUGG-ACG- -5' |
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| 27480 | 5' | -55.5 | NC_005869.1 | + | 16635 | 0.66 | 0.569007 |
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Target: 5'- cGCG-CGGGGuggGGgGCAcCACCGCCgucaGCc -3' miRNA: 3'- -CGUaGUCCC---UCgCGUaGUGGUGGa---CG- -5' |
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| 27480 | 5' | -55.5 | NC_005869.1 | + | 17008 | 0.66 | 0.569007 |
|
Target: 5'- aGCAcgggCAGGGAuaccGUGCGggGCUGCCUGg -3' miRNA: 3'- -CGUa---GUCCCU----CGCGUagUGGUGGACg -5' |
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| 27480 | 5' | -55.5 | NC_005869.1 | + | 14227 | 0.65 | 0.575778 |
|
Target: 5'- aCAUCGGGGugaagaucgacacGCGCAaCuuCCGCCUGg -3' miRNA: 3'- cGUAGUCCCu------------CGCGUaGu-GGUGGACg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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