miRNA: 3'- -CGuAGUCCCUCGCGU----------AG-UGGUGGa--CG- -5'
miRNA: 3'- -CGUa-----GUCCCU--------------CGCGUAG--------------UGG--UGGACG- -5'
miRNA: 3'- cGUAGUCC-CUCGCGua-----GUGGUG-GACG- -5'
miRNA: 3'- -CGUAGUCCCUCGCGUAGUGGUGGACG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27480 | 5' | -55.5 | NC_005869.1 | + | 30211 | 0.67 | 0.481317 |
|
Target: 5'- aGUcUCAGGGAGCGCAggcaucucuuUCaACUACUccaGCa -3' miRNA: 3'- -CGuAGUCCCUCGCGU----------AG-UGGUGGa--CG- -5' |
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| 27480 | 5' | -55.5 | NC_005869.1 | + | 30844 | 0.66 | 0.528865 |
|
Target: 5'- uGCAgcucacCAGGGAccggggggcccgcaGCGCGUCugucacuuucaucuGCCgcACCUGCg -3' miRNA: 3'- -CGUa-----GUCCCU--------------CGCGUAG--------------UGG--UGGACG- -5' |
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| 27480 | 5' | -55.5 | NC_005869.1 | + | 31624 | 0.71 | 0.28958 |
|
Target: 5'- aCAUCAGGuGGGCGCcucgaaaCAUCACaCUGCc -3' miRNA: 3'- cGUAGUCC-CUCGCGua-----GUGGUG-GACG- -5' |
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| 27480 | 5' | -55.5 | NC_005869.1 | + | 31749 | 1.13 | 0.000238 |
|
Target: 5'- cGCAUCAGGGAGCGCAUCACCACCUGCc -3' miRNA: 3'- -CGUAGUCCCUCGCGUAGUGGUGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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