Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27481 | 5' | -60.6 | NC_005869.1 | + | 22422 | 0.71 | 0.159474 |
Target: 5'- aGGCCUGcuacGCGUuccUCGCUCGccACUCCgCCu -3' miRNA: 3'- gCCGGGCa---CGCG---AGCGAGU--UGAGG-GG- -5' |
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27481 | 5' | -60.6 | NC_005869.1 | + | 17443 | 0.71 | 0.146923 |
Target: 5'- uGGCCCG-GCGCcCGCggCGGuuccggugcCUCCCCc -3' miRNA: 3'- gCCGGGCaCGCGaGCGa-GUU---------GAGGGG- -5' |
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27481 | 5' | -60.6 | NC_005869.1 | + | 331 | 0.74 | 0.091413 |
Target: 5'- cCGGCCCGcucgcgGUGUUCGCUCAcACUCgUCu -3' miRNA: 3'- -GCCGGGCa-----CGCGAGCGAGU-UGAGgGG- -5' |
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27481 | 5' | -60.6 | NC_005869.1 | + | 23098 | 0.77 | 0.056193 |
Target: 5'- gGGCCC--GCGCcgCGCUC-ACUCCCCc -3' miRNA: 3'- gCCGGGcaCGCGa-GCGAGuUGAGGGG- -5' |
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27481 | 5' | -60.6 | NC_005869.1 | + | 8308 | 0.77 | 0.051525 |
Target: 5'- gGGCCCGgcgGCGCggGC-CAGCUCCaCCg -3' miRNA: 3'- gCCGGGCa--CGCGagCGaGUUGAGG-GG- -5' |
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27481 | 5' | -60.6 | NC_005869.1 | + | 32289 | 1.1 | 0.000138 |
Target: 5'- cCGGCCCGUGCGCUCGCUCAACUCCCCc -3' miRNA: 3'- -GCCGGGCACGCGAGCGAGUUGAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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