miRNA display CGI


Results 21 - 26 of 26 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27481 5' -60.6 NC_005869.1 + 22422 0.71 0.159474
Target:  5'- aGGCCUGcuacGCGUuccUCGCUCGccACUCCgCCu -3'
miRNA:   3'- gCCGGGCa---CGCG---AGCGAGU--UGAGG-GG- -5'
27481 5' -60.6 NC_005869.1 + 17443 0.71 0.146923
Target:  5'- uGGCCCG-GCGCcCGCggCGGuuccggugcCUCCCCc -3'
miRNA:   3'- gCCGGGCaCGCGaGCGa-GUU---------GAGGGG- -5'
27481 5' -60.6 NC_005869.1 + 331 0.74 0.091413
Target:  5'- cCGGCCCGcucgcgGUGUUCGCUCAcACUCgUCu -3'
miRNA:   3'- -GCCGGGCa-----CGCGAGCGAGU-UGAGgGG- -5'
27481 5' -60.6 NC_005869.1 + 23098 0.77 0.056193
Target:  5'- gGGCCC--GCGCcgCGCUC-ACUCCCCc -3'
miRNA:   3'- gCCGGGcaCGCGa-GCGAGuUGAGGGG- -5'
27481 5' -60.6 NC_005869.1 + 8308 0.77 0.051525
Target:  5'- gGGCCCGgcgGCGCggGC-CAGCUCCaCCg -3'
miRNA:   3'- gCCGGGCa--CGCGagCGaGUUGAGG-GG- -5'
27481 5' -60.6 NC_005869.1 + 32289 1.1 0.000138
Target:  5'- cCGGCCCGUGCGCUCGCUCAACUCCCCc -3'
miRNA:   3'- -GCCGGGCACGCGAGCGAGUUGAGGGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.