Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27483 | 3' | -62.6 | NC_005869.1 | + | 22115 | 0.66 | 0.257905 |
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Target: 5'- uUugACCCCuucgGCUUCuCCGAccGGGAGCu -3' miRNA: 3'- -GugUGGGG----CGGAG-GGCUcaCCCUCGu -5' |
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| 27483 | 3' | -62.6 | NC_005869.1 | + | 23709 | 0.66 | 0.255295 |
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Target: 5'- cCGgGCCaCCGCCgggCUCGGGgucaccgggcgcgGGGGGCGg -3' miRNA: 3'- -GUgUGG-GGCGGa--GGGCUCa------------CCCUCGU- -5' |
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| 27483 | 3' | -62.6 | NC_005869.1 | + | 2064 | 0.66 | 0.252062 |
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Target: 5'- uUAgAUCgCCGCCggCCCGGGggagcggguggagagGGGAGCGg -3' miRNA: 3'- -GUgUGG-GGCGGa-GGGCUCa--------------CCCUCGU- -5' |
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| 27483 | 3' | -62.6 | NC_005869.1 | + | 28597 | 0.66 | 0.245069 |
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Target: 5'- cCGCGCCCCGCCgcugccaccgCCCcucaccuauGUGGGAcccuGCGc -3' miRNA: 3'- -GUGUGGGGCGGa---------GGGcu-------CACCCU----CGU- -5' |
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| 27483 | 3' | -62.6 | NC_005869.1 | + | 24287 | 0.67 | 0.209725 |
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Target: 5'- --aGCCCCGCCgggaCGAG-GaGGAGCAg -3' miRNA: 3'- gugUGGGGCGGagg-GCUCaC-CCUCGU- -5' |
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| 27483 | 3' | -62.6 | NC_005869.1 | + | 21656 | 0.67 | 0.198958 |
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Target: 5'- gACACgugCCCGCgCUCCCcAGcGGGGGCc -3' miRNA: 3'- gUGUG---GGGCG-GAGGGcUCaCCCUCGu -5' |
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| 27483 | 3' | -62.6 | NC_005869.1 | + | 9344 | 0.69 | 0.156294 |
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Target: 5'- uGCugCugCUGCCUCCgCGGGgacgGGGGGCGc -3' miRNA: 3'- gUGugG--GGCGGAGG-GCUCa---CCCUCGU- -5' |
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| 27483 | 3' | -62.6 | NC_005869.1 | + | 17114 | 0.69 | 0.137839 |
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Target: 5'- gGCGCCCC-CCgCCCGAccggccaccgucuccGUGGGGGUg -3' miRNA: 3'- gUGUGGGGcGGaGGGCU---------------CACCCUCGu -5' |
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| 27483 | 3' | -62.6 | NC_005869.1 | + | 32928 | 0.99 | 0.000577 |
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Target: 5'- cUACACaCCCGCCUCCCGAGUGGGAGCAg -3' miRNA: 3'- -GUGUG-GGGCGGAGGGCUCACCCUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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