Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27485 | 5' | -55.3 | NC_005872.1 | + | 7179 | 0.66 | 0.554916 |
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Target: 5'- -aCCC-CCUCC-CAAGGAgCUUuUAGa -3' miRNA: 3'- ggGGGuGGAGGuGUUCCUgGAGuAUC- -5' |
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| 27485 | 5' | -55.3 | NC_005872.1 | + | 23628 | 0.66 | 0.554916 |
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Target: 5'- uCCCCCACCUCUACGcucacgccACCcCAUGc -3' miRNA: 3'- -GGGGGUGGAGGUGUucc-----UGGaGUAUc -5' |
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| 27485 | 5' | -55.3 | NC_005872.1 | + | 464 | 0.67 | 0.478148 |
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Target: 5'- gCCCCCACCUCCcccACAAGaaaaGCCa----- -3' miRNA: 3'- -GGGGGUGGAGG---UGUUCc---UGGaguauc -5' |
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| 27485 | 5' | -55.3 | NC_005872.1 | + | 23464 | 0.68 | 0.426643 |
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Target: 5'- gCCCCUagucaGCCcCCcgauuuCAAGGuCCUCGUAGa -3' miRNA: 3'- -GGGGG-----UGGaGGu-----GUUCCuGGAGUAUC- -5' |
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| 27485 | 5' | -55.3 | NC_005872.1 | + | 6090 | 0.68 | 0.416741 |
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Target: 5'- aCCCUaaugcguuuaACCUCCuuAAGGGCCUCc--- -3' miRNA: 3'- gGGGG----------UGGAGGugUUCCUGGAGuauc -5' |
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| 27485 | 5' | -55.3 | NC_005872.1 | + | 3193 | 0.68 | 0.397365 |
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Target: 5'- gCCCUCaaGCCUCCGCAuaaccuCCUCAUAa -3' miRNA: 3'- -GGGGG--UGGAGGUGUuccu--GGAGUAUc -5' |
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| 27485 | 5' | -55.3 | NC_005872.1 | + | 27716 | 0.68 | 0.387897 |
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Target: 5'- --aUCGCCUCCGCAuuagAGGACCUauUAGg -3' miRNA: 3'- gggGGUGGAGGUGU----UCCUGGAguAUC- -5' |
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| 27485 | 5' | -55.3 | NC_005872.1 | + | 16764 | 0.69 | 0.342832 |
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Target: 5'- uCCUcagCCGCCUCCACAaaGGGaguGCCUUGUAc -3' miRNA: 3'- -GGG---GGUGGAGGUGU--UCC---UGGAGUAUc -5' |
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| 27485 | 5' | -55.3 | NC_005872.1 | + | 2756 | 0.71 | 0.271558 |
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Target: 5'- aCCCCagagcgCGCCUCCugAgaAGGGCCUUAa-- -3' miRNA: 3'- -GGGG------GUGGAGGugU--UCCUGGAGUauc -5' |
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| 27485 | 5' | -55.3 | NC_005872.1 | + | 6796 | 1.11 | 0.000306 |
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Target: 5'- cCCCCCACCUCCACAAGGACCUCAUAGa -3' miRNA: 3'- -GGGGGUGGAGGUGUUCCUGGAGUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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