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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27486 | 5' | -54.5 | NC_005872.1 | + | 25093 | 0.67 | 0.526283 |
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Target: 5'- aUAGCCaaaGCCAcggccuuguuguCCUCCGGAUUagagacaGCCa -3' miRNA: 3'- gAUCGG---UGGU------------GGAGGCCUAAgag----UGG- -5' |
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| 27486 | 5' | -54.5 | NC_005872.1 | + | 419 | 0.67 | 0.515192 |
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Target: 5'- --cGCCACCAUgCUCUGGAggCUuuugagcgCGCCu -3' miRNA: 3'- gauCGGUGGUG-GAGGCCUaaGA--------GUGG- -5' |
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| 27486 | 5' | -54.5 | NC_005872.1 | + | 13787 | 0.67 | 0.493303 |
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Target: 5'- gUAGCCAauaugaACaaggCCGGAgcaaUCUCACCa -3' miRNA: 3'- gAUCGGUgg----UGga--GGCCUa---AGAGUGG- -5' |
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| 27486 | 5' | -54.5 | NC_005872.1 | + | 20662 | 0.73 | 0.238874 |
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Target: 5'- uUAGCCgucuuaACCACCUCCcuaGGAggCUCAgCCu -3' miRNA: 3'- gAUCGG------UGGUGGAGG---CCUaaGAGU-GG- -5' |
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| 27486 | 5' | -54.5 | NC_005872.1 | + | 8110 | 1.11 | 0.000357 |
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Target: 5'- cCUAGCCACCACCUCCGGAUUCUCACCc -3' miRNA: 3'- -GAUCGGUGGUGGAGGCCUAAGAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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