Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27487 | 5' | -52.7 | NC_005872.1 | + | 14923 | 0.66 | 0.680898 |
Target: 5'- cUACGccAGCCaGCGCGGccaauaucgcuauuGCGGCCa -3' miRNA: 3'- cGUGUuuUCGGcCGUGCUuu------------CGCCGG- -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 5609 | 0.66 | 0.656332 |
Target: 5'- aGCACAccauaaucaAGGGCgccaCGGCACGuAAGUGuguGCCg -3' miRNA: 3'- -CGUGU---------UUUCG----GCCGUGCuUUCGC---CGG- -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 10365 | 0.67 | 0.597596 |
Target: 5'- gGCGUAGAauaGGCaaaGGCAUGguGGUGGCUg -3' miRNA: 3'- -CGUGUUU---UCGg--CCGUGCuuUCGCCGG- -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 18683 | 0.67 | 0.597596 |
Target: 5'- cCACGAcAGCCGGUA--GAGGC-GCCa -3' miRNA: 3'- cGUGUUuUCGGCCGUgcUUUCGcCGG- -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 7660 | 0.69 | 0.494748 |
Target: 5'- gGCugAGGAGaaGGCGCGuaa--GGCCg -3' miRNA: 3'- -CGugUUUUCggCCGUGCuuucgCCGG- -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 1652 | 0.69 | 0.472926 |
Target: 5'- gGCGCuuAGGuuGGCugGucuGGCGaCCa -3' miRNA: 3'- -CGUGuuUUCggCCGugCuu-UCGCcGG- -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 3257 | 0.69 | 0.466474 |
Target: 5'- uCACGAGuuGCCgGGCACGccccgggugccguggGGGGCGGCa -3' miRNA: 3'- cGUGUUUu-CGG-CCGUGC---------------UUUCGCCGg -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 26993 | 0.7 | 0.43081 |
Target: 5'- cGCACuugguGCCGcGCACGGAaaauggGGaggaGGCCg -3' miRNA: 3'- -CGUGuuuu-CGGC-CGUGCUU------UCg---CCGG- -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 15063 | 0.72 | 0.310583 |
Target: 5'- gGCACGGAgguuaAGCCuaaGCACGuaauGGCGGCUu -3' miRNA: 3'- -CGUGUUU-----UCGGc--CGUGCuu--UCGCCGG- -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 13841 | 0.75 | 0.203329 |
Target: 5'- gGCACuu-GGCUGGCGCGGcuaugugggccacGGCGGCUa -3' miRNA: 3'- -CGUGuuuUCGGCCGUGCUu------------UCGCCGG- -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 6365 | 0.76 | 0.188753 |
Target: 5'- cCACGAAauuAGCCGGCACGGcacagaaauuGgGGCCa -3' miRNA: 3'- cGUGUUU---UCGGCCGUGCUuu--------CgCCGG- -5' |
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27487 | 5' | -52.7 | NC_005872.1 | + | 11225 | 1.15 | 0.00028 |
Target: 5'- uGCACAAAAGCCGGCACGAAAGCGGCCa -3' miRNA: 3'- -CGUGUUUUCGGCCGUGCUUUCGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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