Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
27488 | 3' | -57 | NC_005872.1 | + | 1318 | 0.68 | 0.325405 |
Target: 5'- aCCACGcccUugGGAGGcCGCGGCCucccuccCGACa -3' miRNA: 3'- cGGUGC---AugCCUCC-GUGUCGGc------GUUG- -5' |
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27488 | 3' | -57 | NC_005872.1 | + | 27795 | 0.7 | 0.230907 |
Target: 5'- aGCCGCGgacACGGAGGCGCAcaauauauuuGCCa---- -3' miRNA: 3'- -CGGUGCa--UGCCUCCGUGU----------CGGcguug -5' |
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27488 | 3' | -57 | NC_005872.1 | + | 13324 | 0.71 | 0.195152 |
Target: 5'- aGCCGCGUauACGGccccaccugccacAGcCACAGCCGCGGa -3' miRNA: 3'- -CGGUGCA--UGCC-------------UCcGUGUCGGCGUUg -5' |
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27488 | 3' | -57 | NC_005872.1 | + | 13686 | 1.12 | 0.000153 |
Target: 5'- aGCCACGUACGGAGGCACAGCCGCAACg -3' miRNA: 3'- -CGGUGCAUGCCUCCGUGUCGGCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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