Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27489 | 5' | -50.7 | NC_005872.1 | + | 4299 | 0.66 | 0.797497 |
Target: 5'- uGGCUuguuGGCUCuAGUGGCAccuCCAguuuagcuuuucgaaACACGGu -3' miRNA: 3'- -CCGA----UCGAG-UUACUGUc--GGU---------------UGUGCC- -5' |
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27489 | 5' | -50.7 | NC_005872.1 | + | 25098 | 0.66 | 0.793291 |
Target: 5'- aGGCUAgGCUUGAUGGCua-CggUugGGa -3' miRNA: 3'- -CCGAU-CGAGUUACUGucgGuuGugCC- -5' |
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27489 | 5' | -50.7 | NC_005872.1 | + | 2985 | 0.66 | 0.790115 |
Target: 5'- uGGCUAGCcagacggaaagaacUaCAgugucuggGUGAgGGCUGACGCGGu -3' miRNA: 3'- -CCGAUCG--------------A-GU--------UACUgUCGGUUGUGCC- -5' |
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27489 | 5' | -50.7 | NC_005872.1 | + | 13674 | 0.66 | 0.782639 |
Target: 5'- aGGCacAGCcgCAAcGGCAcccGCCAACACGa -3' miRNA: 3'- -CCGa-UCGa-GUUaCUGU---CGGUUGUGCc -5' |
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27489 | 5' | -50.7 | NC_005872.1 | + | 20667 | 0.67 | 0.703757 |
Target: 5'- cGGCaUAGC-CAcaucuuuggugAUGACuuuGUCGGCACGGc -3' miRNA: 3'- -CCG-AUCGaGU-----------UACUGu--CGGUUGUGCC- -5' |
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27489 | 5' | -50.7 | NC_005872.1 | + | 22757 | 0.68 | 0.668431 |
Target: 5'- cGGCUcAGCUCAcgGACAucauauuucaCCGGCACa- -3' miRNA: 3'- -CCGA-UCGAGUuaCUGUc---------GGUUGUGcc -5' |
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27489 | 5' | -50.7 | NC_005872.1 | + | 16085 | 0.69 | 0.608926 |
Target: 5'- cGGCUAgGCUguuuaGAUGACAGCCu-UACGu -3' miRNA: 3'- -CCGAU-CGAg----UUACUGUCGGuuGUGCc -5' |
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27489 | 5' | -50.7 | NC_005872.1 | + | 23685 | 0.7 | 0.561663 |
Target: 5'- aGGCUAGCUUuau--CGGCUAugAUGGc -3' miRNA: 3'- -CCGAUCGAGuuacuGUCGGUugUGCC- -5' |
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27489 | 5' | -50.7 | NC_005872.1 | + | 8388 | 0.73 | 0.37845 |
Target: 5'- -aCUAGCguaaAAaGACAGCCAACACGa -3' miRNA: 3'- ccGAUCGag--UUaCUGUCGGUUGUGCc -5' |
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27489 | 5' | -50.7 | NC_005872.1 | + | 13847 | 0.74 | 0.323988 |
Target: 5'- uGGCUGGCgcggCuAUGugGGCCAcgGCGGc -3' miRNA: 3'- -CCGAUCGa---GuUACugUCGGUugUGCC- -5' |
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27489 | 5' | -50.7 | NC_005872.1 | + | 13849 | 1.13 | 0.000663 |
Target: 5'- aGGCUAGCUCAAUGACAGCCAACACGGc -3' miRNA: 3'- -CCGAUCGAGUUACUGUCGGUUGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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