Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2749 | 3' | -57.3 | NC_001491.2 | + | 134075 | 0.66 | 0.850955 |
Target: 5'- -gUCGGCCAGCGacuCCGaugacgcggcUAAAGUAGGa- -3' miRNA: 3'- caGGCCGGUCGC---GGC----------AUUUCAUCCga -5' |
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2749 | 3' | -57.3 | NC_001491.2 | + | 80517 | 0.66 | 0.842949 |
Target: 5'- cGUCCGGCgCGGCgguuGCCagcacgGGAGUAGuGCg -3' miRNA: 3'- -CAGGCCG-GUCG----CGGca----UUUCAUC-CGa -5' |
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2749 | 3' | -57.3 | NC_001491.2 | + | 146345 | 0.67 | 0.809081 |
Target: 5'- aGUCCGGCCucccCGCCucGUcucucGGGUAGGCc -3' miRNA: 3'- -CAGGCCGGuc--GCGG--CAu----UUCAUCCGa -5' |
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2749 | 3' | -57.3 | NC_001491.2 | + | 82938 | 0.67 | 0.800191 |
Target: 5'- --gCGGCgAGCGCCGUccugcGGUugGGGCa -3' miRNA: 3'- cagGCCGgUCGCGGCAuu---UCA--UCCGa -5' |
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2749 | 3' | -57.3 | NC_001491.2 | + | 147397 | 0.67 | 0.798394 |
Target: 5'- cUCCGGCCugGGcCGCCGgcuccagGGGCUc -3' miRNA: 3'- cAGGCCGG--UC-GCGGCauuuca-UCCGA- -5' |
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2749 | 3' | -57.3 | NC_001491.2 | + | 57849 | 0.68 | 0.743959 |
Target: 5'- cUCUGGCCAugucccCGUCGUAGuccuuGGUGGGCg -3' miRNA: 3'- cAGGCCGGUc-----GCGGCAUU-----UCAUCCGa -5' |
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2749 | 3' | -57.3 | NC_001491.2 | + | 79824 | 0.68 | 0.734189 |
Target: 5'- cUCUGG-CAGCucGCCGUuuccuAGGUAGGCg -3' miRNA: 3'- cAGGCCgGUCG--CGGCAu----UUCAUCCGa -5' |
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2749 | 3' | -57.3 | NC_001491.2 | + | 30874 | 0.74 | 0.375717 |
Target: 5'- -aCCGGCUauGGCGCgCGUguuggucagcgggGAAGUAGGCUg -3' miRNA: 3'- caGGCCGG--UCGCG-GCA-------------UUUCAUCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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