Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27490 | 3' | -45.9 | NC_005872.1 | + | 25468 | 0.67 | 0.947433 |
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Target: 5'- uACGugGCgGACcgcauGGgcGAUAGACACg -3' miRNA: 3'- -UGUugCGgUUGu----UCauCUGUUUGUGa -5' |
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| 27490 | 3' | -45.9 | NC_005872.1 | + | 13565 | 0.67 | 0.934431 |
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Target: 5'- gACcACGCCAACAAGgcacccaAGACGccaccaaagccuauGACACc -3' miRNA: 3'- -UGuUGCGGUUGUUCa------UCUGU--------------UUGUGa -5' |
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| 27490 | 3' | -45.9 | NC_005872.1 | + | 14985 | 0.68 | 0.909587 |
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Target: 5'- uGCAACGCCGACAcc--GuCAAAUACg -3' miRNA: 3'- -UGUUGCGGUUGUucauCuGUUUGUGa -5' |
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| 27490 | 3' | -45.9 | NC_005872.1 | + | 16673 | 0.68 | 0.902044 |
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Target: 5'- gGCAugG-CAAUAGGUAGcuaccCAGACGCUg -3' miRNA: 3'- -UGUugCgGUUGUUCAUCu----GUUUGUGA- -5' |
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| 27490 | 3' | -45.9 | NC_005872.1 | + | 22060 | 0.73 | 0.655292 |
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Target: 5'- uAUGugGCCGGCGuguuGGUGGACAAGgACa -3' miRNA: 3'- -UGUugCGGUUGU----UCAUCUGUUUgUGa -5' |
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| 27490 | 3' | -45.9 | NC_005872.1 | + | 14134 | 1.08 | 0.004522 |
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Target: 5'- aACAACGCCAACAAGUAGACAAACACUu -3' miRNA: 3'- -UGUUGCGGUUGUUCAUCUGUUUGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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