Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27493 | 5' | -46.8 | NC_005872.1 | + | 13847 | 0.66 | 0.950339 |
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Target: 5'- uGGCuGGCGCGGcUAug-UGGGCCACgGc -3' miRNA: 3'- -CCG-UUGUGCC-AUuaaACUUGGUGgUa -5' |
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| 27493 | 5' | -46.8 | NC_005872.1 | + | 19155 | 0.67 | 0.891227 |
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Target: 5'- gGGCAgugacgGCACGGUuaacUUGAaauACCACUAc -3' miRNA: 3'- -CCGU------UGUGCCAuua-AACU---UGGUGGUa -5' |
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| 27493 | 5' | -46.8 | NC_005872.1 | + | 23514 | 0.69 | 0.836862 |
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Target: 5'- cGGCuauCACGGUGAUU--AACCACa-- -3' miRNA: 3'- -CCGuu-GUGCCAUUAAacUUGGUGgua -5' |
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| 27493 | 5' | -46.8 | NC_005872.1 | + | 10457 | 0.7 | 0.749803 |
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Target: 5'- aGCGGCgGCGGUGGUggGAGCaaCACCAc -3' miRNA: 3'- cCGUUG-UGCCAUUAaaCUUG--GUGGUa -5' |
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| 27493 | 5' | -46.8 | NC_005872.1 | + | 19037 | 0.71 | 0.738074 |
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Target: 5'- uGGUAACugGGUGAUagauauACCACCc- -3' miRNA: 3'- -CCGUUGugCCAUUAaacu--UGGUGGua -5' |
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| 27493 | 5' | -46.8 | NC_005872.1 | + | 20372 | 1 | 0.013738 |
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Target: 5'- uGGCAACACGGUAAUUUGA-CCACCAUa -3' miRNA: 3'- -CCGUUGUGCCAUUAAACUuGGUGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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