Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27496 | 3' | -51.4 | NC_005872.1 | + | 9591 | 0.66 | 0.685135 |
Target: 5'- uAAAGCGagagGUCCuccGgACCACGCCGAg -3' miRNA: 3'- uUUUUGCa---CGGGu--UgUGGUGCGGUUa -5' |
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27496 | 3' | -51.4 | NC_005872.1 | + | 16863 | 0.66 | 0.660964 |
Target: 5'- -------aGCCCAGCcucgcCCGCGCCAAa -3' miRNA: 3'- uuuuugcaCGGGUUGu----GGUGCGGUUa -5' |
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27496 | 3' | -51.4 | NC_005872.1 | + | 27954 | 0.66 | 0.660964 |
Target: 5'- cAAAAugGUGCUUAAggucgaaauaaGCCGCGCCAc- -3' miRNA: 3'- -UUUUugCACGGGUUg----------UGGUGCGGUua -5' |
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27496 | 3' | -51.4 | NC_005872.1 | + | 18761 | 0.68 | 0.576042 |
Target: 5'- -------aGCCCGACGCCACaGUCAAa -3' miRNA: 3'- uuuuugcaCGGGUUGUGGUG-CGGUUa -5' |
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27496 | 3' | -51.4 | NC_005872.1 | + | 23192 | 0.68 | 0.552106 |
Target: 5'- uAAAGCGggagGUCCuccGgACCACGCCGAg -3' miRNA: 3'- uUUUUGCa---CGGGu--UgUGGUGCGGUUa -5' |
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27496 | 3' | -51.4 | NC_005872.1 | + | 13900 | 0.68 | 0.540249 |
Target: 5'- ----cCGUgGCCCAcauaGCCGCGCCAGc -3' miRNA: 3'- uuuuuGCA-CGGGUug--UGGUGCGGUUa -5' |
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27496 | 3' | -51.4 | NC_005872.1 | + | 11038 | 0.75 | 0.229651 |
Target: 5'- gGGAGGCGUGUCCAugucaagcccauAgGCCACGCCGu- -3' miRNA: 3'- -UUUUUGCACGGGU------------UgUGGUGCGGUua -5' |
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27496 | 3' | -51.4 | NC_005872.1 | + | 26359 | 0.91 | 0.017179 |
Target: 5'- gAAAAACGUGCCCAACA-CACGCCAAUg -3' miRNA: 3'- -UUUUUGCACGGGUUGUgGUGCGGUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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