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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27496 | 5' | -51.8 | NC_005872.1 | + | 2528 | 0.66 | 0.676217 |
Target: 5'- --cUUCACauGCCGUGGAgGCGUGa- -3' miRNA: 3'- ccuGAGUGauCGGCACCUgUGCAUaa -5' |
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27496 | 5' | -51.8 | NC_005872.1 | + | 7629 | 0.7 | 0.456924 |
Target: 5'- aGGGCUCug-AGCCGgagGaGAUACGUGUg -3' miRNA: 3'- -CCUGAGugaUCGGCa--C-CUGUGCAUAa -5' |
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27496 | 5' | -51.8 | NC_005872.1 | + | 26322 | 1.08 | 0.001123 |
Target: 5'- cGGACUCACUAGCCGUGGACACGUAUUc -3' miRNA: 3'- -CCUGAGUGAUCGGCACCUGUGCAUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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