Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27508 | 5' | -49.4 | NC_005879.1 | + | 7223 | 0.65 | 0.932033 |
Target: 5'- --aAGCGCgGCcugcaaucggaccGCCGAUGAuGAUUGCg -3' miRNA: 3'- ugcUUGCGgCG-------------CGGUUGCUuUUAACG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 35136 | 0.66 | 0.920418 |
Target: 5'- gGCGAGCGCacaCGCaaGUCAACuGAAGcUGCg -3' miRNA: 3'- -UGCUUGCG---GCG--CGGUUGcUUUUaACG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 26523 | 0.66 | 0.920418 |
Target: 5'- uUGAcacGCGCgGCGgCGAUGAAGAUucaUGUa -3' miRNA: 3'- uGCU---UGCGgCGCgGUUGCUUUUA---ACG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 14030 | 0.67 | 0.86807 |
Target: 5'- --uGACGCCGaCGgCAACGAagucgAAGUUGUu -3' miRNA: 3'- ugcUUGCGGC-GCgGUUGCU-----UUUAACG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 18877 | 0.68 | 0.850517 |
Target: 5'- cAUGAugGCCGCaGCCGACa-------- -3' miRNA: 3'- -UGCUugCGGCG-CGGUUGcuuuuaacg -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 31786 | 0.68 | 0.835724 |
Target: 5'- cCGAuACGCCGCGCugucuccgCAGCGAAucgccuuuuuugUGCg -3' miRNA: 3'- uGCU-UGCGGCGCG--------GUUGCUUuua---------ACG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 8731 | 0.69 | 0.812388 |
Target: 5'- -aGAGCGCCGaCuaCAGCGAAAcguccaUGCa -3' miRNA: 3'- ugCUUGCGGC-GcgGUUGCUUUua----ACG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 3716 | 0.69 | 0.770842 |
Target: 5'- uGCGAgccuGCGCUuuuuCGCCAACGAAug--GCg -3' miRNA: 3'- -UGCU----UGCGGc---GCGGUUGCUUuuaaCG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 39491 | 0.7 | 0.749045 |
Target: 5'- gGCGAAgGCCGaUGCCAAgGggGc--GCa -3' miRNA: 3'- -UGCUUgCGGC-GCGGUUgCuuUuaaCG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 39288 | 0.71 | 0.65758 |
Target: 5'- gACaGACGCC-CGuCCGACGggGGUUcGCg -3' miRNA: 3'- -UGcUUGCGGcGC-GGUUGCuuUUAA-CG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 21306 | 0.72 | 0.645888 |
Target: 5'- cCGAGcCGCCGCuauucaGCCAACGAGGca-GCg -3' miRNA: 3'- uGCUU-GCGGCG------CGGUUGCUUUuaaCG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 27473 | 0.74 | 0.51881 |
Target: 5'- uGCGGugGCCGauagcuuaggcaUGCCAugGGAGuggUGCg -3' miRNA: 3'- -UGCUugCGGC------------GCGGUugCUUUua-ACG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 13850 | 0.76 | 0.415615 |
Target: 5'- gACG-ACGCCaaacccuGCGCCGACGGAguggcagguguuugcAGUUGCg -3' miRNA: 3'- -UGCuUGCGG-------CGCGGUUGCUU---------------UUAACG- -5' |
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27508 | 5' | -49.4 | NC_005879.1 | + | 8763 | 1.11 | 0.001962 |
Target: 5'- uACGAACGCCGCGCCAACGAAAAUUGCc -3' miRNA: 3'- -UGCUUGCGGCGCGGUUGCUUUUAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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